Tat (NM_146214) Mouse Tagged ORF Clone
CAT#: MR207246
- TrueORF®
Tat (Myc-DDK-tagged) - Mouse tyrosine aminotransferase (Tat), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_146214" in other vectors (4)
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CNY 3,656.00
CNY 4,090.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207246 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCTACGTAATCCAGACGAATGTCAATGACAGCCTACCTTCAGTGCTGGATGTTCGCGTCAATA TTGGCGGGAGAAGCTCGGTGCAAGGGAGAGCGAAGGGCAGGAAGGCCAGGTGGAATGTGAGGCCCTCTGA CATGTCCAATAAAACCTTCAATCCCATCCGAGCCATTGTGGACAACATGAAGGTGAAGCCGAATCCGAAC AAAACCGTGATCTCTCTGTCAATTGGGGACCCTACTGTGTTTGGGAACCTGCCCACAGACCCTGAAGTGA CCCAAGCCATGAAGGATGCCCTGGACTCCGGGAAGTACAATGGCTATGCCCCATCTATCGGCTACCTATC CAGTCGGGAGGAGGTCGCTTCCTATTACCACTGTCCTGAGGCTCCTCTGGAAGCTAAGGATGTCATTCTG ACGAGTGGCTGCAGTCAGGCCATTGAGCTGTGTCTAGCCGTGTTGGCCAATCCTGGACAGAACATCCTCA TTCCGAGGCCCGGGTTTTCCCTCTACAGGACATTGGCTGAGTCTATGGGGATTGAGGTCAAGCTCTACAA TCTATTGCCTGAGAAGTCTTGGGAAATTGATCTAAAACAACTGGAATCTCTGATCGACGAAAAAACAGCT TGTCTCGTGGTCAACAACCCGTCCAATCCCTGTGGCTCTGTGTTCAGTAAGCGGCACCTTCAGAAGATTT TGGCAGTGGCTGAAAGGCAATGCGTCCCCATCTTAGCCGATGAGATCTATGGTGACATGGTGTTTTCAGA TTGCAAATATGAACCAATGGCCACCCTCAGCACCAATGTCCCCATCCTGTCCTGTGGTGGGCTGGCCAAG CGCTGGCTGGTTCCTGGCTGGAGGCTGGGCTGGATCCTTATCCATGATCGAAGAGACATTTTTGGCAATG AGATTCGGGACGGGCTGGTGAAGCTGAGTCAGCGGATCCTGGGCCCGTGCACCATCGTCCAAGGTGCCCT GAAGAGCATCCTTCAGCGCACCCCTCAGGAGTTCTACCAGGACACTTTAAGCTTCCTTAAGTCCAATGCG GACCTCTGCTATGGGGCGTTGTCTGCCATTCCTGGACTCCAGCCAGTCCGCCCATCTGGAGCCATGTACC TTATGGTGGGAATTGAGATGGAGCACTTCCCAGAATTTGAGAATGACGTGGAATTCACAGAGCGGTTAAT TGCGGAGCAGTCTGTCCACTGCCTCCCAGCCACGTGCTTCGAGTACCCAAATTTCTTCCGGGTTGTCATC ACAGTCCCCGAGGTGATGATGCTGGAGGCTTGTAGCCGGATCCAGGAGTTCTGTGAACAGCACTACCACT GTGCTGAAGGCAGCCAGGAGGAGTGTGACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207246 protein sequence
Red=Cloning site Green=Tags(s) MDSYVIQTNVNDSLPSVLDVRVNIGGRSSVQGRAKGRKARWNVRPSDMSNKTFNPIRAIVDNMKVKPNPN KTVISLSIGDPTVFGNLPTDPEVTQAMKDALDSGKYNGYAPSIGYLSSREEVASYYHCPEAPLEAKDVIL TSGCSQAIELCLAVLANPGQNILIPRPGFSLYRTLAESMGIEVKLYNLLPEKSWEIDLKQLESLIDEKTA CLVVNNPSNPCGSVFSKRHLQKILAVAERQCVPILADEIYGDMVFSDCKYEPMATLSTNVPILSCGGLAK RWLVPGWRLGWILIHDRRDIFGNEIRDGLVKLSQRILGPCTIVQGALKSILQRTPQEFYQDTLSFLKSNA DLCYGALSAIPGLQPVRPSGAMYLMVGIEMEHFPEFENDVEFTERLIAEQSVHCLPATCFEYPNFFRVVI TVPEVMMLEACSRIQEFCEQHYHCAEGSQEECDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_146214 |
ORF Size | 1365 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146214.3 |
RefSeq Size | 2377 bp |
RefSeq ORF | 1365 bp |
Locus ID | 234724 |
UniProt ID | Q8QZR1 |
MW | 50.6 kDa |
Gene Summary | This gene encodes a liver-specific mitochondrial enzyme that catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Regulated by glucocorticoid and polypeptide hormones, this gene's expression is affected by deletion of a regulatory region near the albino locus on chromosome 7. Mutations in this gene cause tyrosinemia type II in humans. [provided by RefSeq, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204147 | Tat (untagged) - Mouse tyrosine aminotransferase (Tat), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,656.00 |
|
MG207246 | Tat (tGFP-tagged) - Mouse tyrosine aminotransferase (Tat) |
CNY 4,470.00 |
|
MR207246L3 | Lenti ORF clone of Tat (Myc-DDK-tagged) - Mouse tyrosine aminotransferase (Tat), nuclear gene encoding mitochondrial protein |
CNY 5,990.00 |
|
MR207246L4 | Lenti ORF clone of Tat (mGFP-tagged) - Mouse tyrosine aminotransferase (Tat), nuclear gene encoding mitochondrial protein |
CNY 5,990.00 |