Acot2 (NM_134188) Mouse Tagged ORF Clone
CAT#: MR207232
- TrueORF®
Acot2 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 2 (Acot2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_134188" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA571646; MTE-I; Mte1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207232 representing NM_134188
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCTCGTCTTTCGCTGTCCTGAGAGCAAGCAGGTTGTGCCAACAGGATTGGAAGAGCTGGGCGC GGCTGTTTGTACCTCCGCCGCTCAGCACTGGTGGCCGGACCACTTGGGCGCGGACAAATGCGACGCTGAG CGTAGAGCCCGAGGGCCGCAGCTGCTGGGACGAGCCGCTGAGCATCGCCGTGCGCGGCCTGGCCCCCGAG CAGCCCGTCACGCTGCGCTCTGCCTTGCGCGACGAGAAGGGCGCGCTCTTCCGAGCCCACGCGCGCTACC GCGCCGACGCTGGCGGCGAGCTGAACCTGGCGCGCGCGCCCGCGCTGGGCGGCAGCTTCTCGGGGCTCGA GCCCATGGGGCTGCTCTGGGCCATGGAGCCCGAACGGCCTCTCTGGCGCCTGATCAAGCGCGACGTGCAG ACGCCCTTCTTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGATGGCGGGCAGCGGCTGGCGCAGG CGGTGCACGAGCGTCACTTCTTGGCTCCCGGGGTGCGGCGCGTGCCCGTACGAGAGGGCCGGGTGCGCGC CACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTCCTGGGATCATAGACCTTTTTGGAGTTGGAGGTGGC CTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTGATGGCTCTGGCTTATTACAACT ACGACGACCTCCCCAAGAGCATAGAAACCATGCACATGGAGTACTTTGAAGAAGCCGTGAACTACCTGCG CAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGCTGCTTGGGATTTCCAAAGGGGGTGAACTTGGCCTT GCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGCTGTGGTCATCAATGGCTCCGTGGCTGCTGTTGGGA ACACCATCTCCTACAAGGATGAGACTATACCCCCTGTGTCCCTTCTGAGAAACCAAGTCAAAATGACCAA AGATGGTCTCTTGGATGTCGTGGAAGCTCTGCAAAGCCCTCTGGTAGACAAGAAGAGCTTCATTCCCGTG GAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGGTCAGGACGACCACAACTGGAAGAGCGAGTTCTATG CTGATGAGATCTCCAAACGCCTGCAGGCGCACGGGAAGGAGAAGCCCCAGATCATCTGCTACCCAGCAGC AGGGCATTACATCGAGCCCCCTTACTTCCCGCTGTGCAGCGCTGGCATGCACCTCCTGGTGGGTGCCAAC ATCACCTTTGGAGGGGAGCCCAGGGCTCATGCCGTGGCCCAGGTGGACGCATGGCAGCAGCTCCAGACTT TCTTCCACAAACAGTTGGGTAGCAAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207232 representing NM_134188
Red=Cloning site Green=Tags(s) MVASSFAVLRASRLCQQDWKSWARLFVPPPLSTGGRTTWARTNATLSVEPEGRSCWDEPLSIAVRGLAPE QPVTLRSALRDEKGALFRAHARYRADAGGELNLARAPALGGSFSGLEPMGLLWAMEPERPLWRLIKRDVQ TPFLVELEVLDGHEPDGGQRLAQAVHERHFLAPGVRRVPVREGRVRATLFLPPEPGPFPGIIDLFGVGGG LLEYRASLLAGKGFAVMALAYYNYDDLPKSIETMHMEYFEEAVNYLRSHPEVKGPGIGLLGISKGGELGL AMASFLKGITAAVVINGSVAAVGNTISYKDETIPPVSLLRNQVKMTKDGLLDVVEALQSPLVDKKSFIPV ERSDTTFLFLVGQDDHNWKSEFYADEISKRLQAHGKEKPQIICYPAAGHYIEPPYFPLCSAGMHLLVGAN ITFGGEPRAHAVAQVDAWQQLQTFFHKQLGSKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134188 |
ORF Size | 1359 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134188.3, NP_598949.3 |
RefSeq Size | 2215 bp |
RefSeq ORF | 1362 bp |
Locus ID | 171210 |
UniProt ID | Q9QYR9 |
MW | 49.7 kDa |
Gene Summary | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:25114170). Acyl-coenzyme A thioesterase 2/ACOT2 displays higher activity toward long chain acyl CoAs (C14-C20) (PubMed:25114170). The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria (PubMed:25114170).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216033 | Acot2 (untagged) - Mouse acyl-CoA thioesterase 2 (Acot2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 5,800.00 |
|
MG207232 | Acot2 (tGFP-tagged) - Mouse acyl-CoA thioesterase 2 (Acot2) |
CNY 3,710.00 |
|
MR207232L3 | Lenti ORF clone of Acot2 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 2 (Acot2), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
MR207232L4 | Lenti ORF clone of Acot2 (mGFP-tagged) - Mouse acyl-CoA thioesterase 2 (Acot2), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |