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Adam15 (BC009132) Mouse Tagged ORF Clone
CAT＃: MR207150
- TrueORF®
Adam15 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete
ORF Plasmid: tGFP
"BC009132" in other vectors (4)
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CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MDC15, metargidin |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207150 representing BC009132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTGGCGCTGCTCTGGGCTCTGGGACTCCTGGGCGCGGGCAGCCCTCGGCCCTCCCCGCCGCTGC CAAATATAGGAGGCACTGAGGAAGAGCAGCAAGCCAGCCCAGAGAGGACGCTGAGTGGATCCATGGAGAG CCGGGTTGTTCAGGACAGCCCCCCAATGAGCCTAGCAGACGTGCTTCAGACTGGTTTACCTGAGGCCCTG AGGATTTCCTTGGAGCTGGACAGTGAGAGTCATGTCCTGGAGCTTCTACAAAATAGAGATCTAATCCCTG GCCGCCCAACTCTGGTGTGGTACCAGCCTGATGGCACCCGAATGGTCAGCGAGGGCTACAGTCTAGAAAA CTGCTGCTACCGAGGACGAGTGCAGGGCCACCCCAGCTCCTGGGTGTCCCTCTGTGCCTGCTCTGGGATC AGGGGGCTCATTGTCCTGTCCCCAGAGAGAGGCTATACACTGGAGCTGGGCCCTGGGGACCTTCAGCGTC CTGTCATTTCTCGGATCCAAGACCACCTGTTGCTGGGCCACACCTGTGCCCCAAGCTGGCATGCCTCTGT GCCCACTCGGGCAGGACCAGACCTCCTTCTGGAACAGCATCACGCTCACAGGCTTAAGCGAGATGTAGTA ACAGAGACGAAAATTGTGGAGTTGGTGATTGTGGCTGATAATTCAGAGGTCAGAAAGTACCCTGACTTCC AACAACTGCTGAACCGGACACTAGAAGCGGCTCTCTTGCTAGACACGTTCTTCCAGCCCTTGAATGTCCG GGTAGCCCTTGTGGGCCTAGAGGCATGGACCCAGCACAACCTGATAGAAATGAGCTCCAACCCAGCTGTC CTGCTAGACAACTTCCTCCGCTGGCGCCGGACAGACTTGCTGCCTCGACTGCCCCATGACAGTGCCCAAC TGGTGACTGTAACTTCCTTCTCTGGTCCCATGGTGGGCATGGCCATTCAGAATTCCATCTGTTCCCCTGA CTTCTCCGGAGGTGTGAATATGGACCACTCCACAAGCATCTTAGGCGTTGCCTCCTCGATTGCCCATGAA TTGGGCCACAGTCTGGGTTTGGACCATGATTCTCCCGGGCACAGCTGTCCCTGTCCAGGTCCAGCCCCGG CTAAGAGCTGCATCATGGAGGCCTCCACAGACTTCCTACCAGGTTTGAACTTCAGCAACTGCAGCCGACA GGCCCTGGAAAAGGCCCTCCTGGAAGGAATGGGCAGCTGCCTCTTCGAACGGCACCCACCAGGTGACCTG CCTGGCCCAGGAGATGGAAGCTTGCCGCTGGTGGTGCCCTCCAGGCCAGCTCCACCACCCCCTGCAGCAT CTTCGCTCTACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207150 representing BC009132
Red=Cloning site Green=Tags(s) MRLALLWALGLLGAGSPRPSPPLPNIGGTEEEQQASPERTLSGSMESRVVQDSPPMSLADVLQTGLPEAL RISLELDSESHVLELLQNRDLIPGRPTLVWYQPDGTRMVSEGYSLENCCYRGRVQGHPSSWVSLCACSGI RGLIVLSPERGYTLELGPGDLQRPVISRIQDHLLLGHTCAPSWHASVPTRAGPDLLLEQHHAHRLKRDVV TETKIVELVIVADNSEVRKYPDFQQLLNRTLEAALLLDTFFQPLNVRVALVGLEAWTQHNLIEMSSNPAV LLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAHE LGHSLGLDHDSPGHSCPCPGPAPAKSCIMEASTDFLPGLNFSNCSRQALEKALLEGMGSCLFERHPPGDL PGPGDGSLPLVVPSRPAPPPPAASSLYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC009132 |
| ORF Size | 1344 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC009132.1 |
| RefSeq Size | 1704 bp |
| RefSeq ORF | 1346 bp |
| Locus ID | 11490 |
| MW | 62.5 kDa |
| Gene Summary | This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is prominently expressed in vascular cells, the endocardium, hypertrophic cells in developing bone, and specific areas of hippocampus and cerebellum. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Mice lacking the encoded protein have increased bone mass resulting from osteoblast proliferation, and exhibit reduced neovascularization in a mouse model for retinopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200662 | Adam15 (untagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete, (10ug) |
CNY 3,656.00 |
|
| MG207150 | Adam15 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete |
CNY 4,940.00 |
|
| MR207150L3 | Lenti ORF clone of Adam15 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete |
CNY 6,370.00 |
|
| MR207150L4 | Lenti ORF clone of Adam15 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 15 (metargidin) (cDNA clone MGC:11753 IMAGE:3152945), complete |
CNY 6,370.00 |
