Efemp2 (NM_001164352) Mouse Tagged Lenti ORF Clone
CAT#: MR207074L3
- LentiORF®
Lenti ORF clone of Efemp2 (Myc-DDK-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2
"NM_001164352" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 6,650.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 0610011K11Rik; Fbln4; MBP1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR207074).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001164352 |
ORF Size | 1332 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164352.1, NP_001157824.1 |
RefSeq Size | 1802 bp |
RefSeq ORF | 1389 bp |
Locus ID | 58859 |
Gene Summary | Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta (PubMed:16478991, PubMed:19855011, PubMed:20019329, PubMed:26486174, PubMed:26711913, PubMed:28508064). In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (PubMed:26690653, PubMed:26711913, PubMed:26220971, PubMed:26178373). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction (PubMed:17324935). Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling (PubMed:23636094).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218345 | Efemp2 (untagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2, (10ug) |
CNY 6,940.00 |
|
MR207074 | Efemp2 (Myc-DDK-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2 |
CNY 3,656.00 |
|
MR207074L4 | Lenti ORF clone of Efemp2 (mGFP-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2 |
CNY 6,650.00 |