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Efemp2 (NM_001164352) Mouse Untagged Clone
CAT＃: MC218345
Efemp2 (untagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2, (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610011K11Rik; Fbln4; MBP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218345 representing NM_001164352
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGGTGCTGTGGCAGAAACCCCTGACTTCTGCCCACCACCTCCCAGCCTCAGGATGCTCCCTTTTG CCTCCTGCCTCCCCGGGTCTTTGCTGCTCTGGGCGTTTCTGCTGTTGCTCTTGGGAGCAGCGTCCCCACA GGATCCCGAGGAGCCGGACAGCTACACGGAATGCACAGATGGCTATGAGTGGGATGCAGACAGCCAGCAC TGCCGGGATGTCAACGAGTGCCTGACCATCCCGGAGGCTTGCAAGGGTGAGATGAAATGCATCAACCACT ACGGGGGTTATTTGTGTCTGCCTCGCTCTGCTGCCGTCATCAGTGATCTCCATGGTGAAGGACCTCCACC GCCAGCGGCCCATGCTCAACAACCAAACCCTTGCCCGCAGGGCTACGAGCCTGATGAACAGGAGAGCTGT GTGGATGTGGACGAGTGTACCCAGGCTTTGCATGACTGTCGCCCTAGTCAGGACTGCCATAACCTTCCTG GCTCCTACCAGTGCACCTGCCCTGATGGTTACCGAAAAATTGGACCCGAATGTGTGGACATAGATGAGTG TCGTTACCGCTATTGCCAGCATCGATGTGTGAACCTGCCGGGCTCTTTTCGATGCCAGTGTGAGCCAGGC TTCCAGTTGGGACCTAACAACCGCTCTTGTGTGGATGTGAATGAGTGTGACATGGGAGCCCCATGTGAGC AGCGCTGCTTCAACTCCTATGGGACCTTCCTGTGTCGCTGTAACCAGGGCTATGAGCTGCACCGGGATGG CTTCTCCTGCAGCGATATCGATGAGTGCGGCTACTCCAGTTACCTCTGCCAGTACCGCTGTGTCAACGAG CCAGGCCGATTCTCCTGTCACTGCCCACAAGGCTACCAGCTGCTGGCTACAAGGCTCTGCCAAGATATTG ACGAGTGTGAAACAGGTGCACACCAATGTTCTGAGGCCCAAACCTGTGTCAACTTCCATGGGGGTTACCG CTGTGTGGACACCAACCGTTGTGTGGAGCCCTATGTCCAAGTGTCAGACAACCGCTGCCTCTGCCCTGCC TCCAATCCCCTTTGTCGAGAGCAGCCTTCATCCATTGTGCACCGCTACATGAGCATCACCTCAGAGCGAA GTGTGCCTGCTGACGTGTTTCAGATCCAGGCAACCTCTGTCTACCCTGGTGCCTACAATGCCTTTCAGAT CCGTTCTGGAAACACACAGGGGGACTTCTACATTAGGCAAATCAACAATGTCAGCGCCATGCTGGTCCTC GCCAGGCCAGTGACGGGACCCCGGGAGTACGTGCTGGACCTGGAGATGGTCACCATGAATTCCCTTATGA GCTACCGGGCCAGCTCTGTACTGAGACTCACGGTCTTTGTGGGAGCCTATACCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001164352 |
| Insert Size | 1389 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164352.1, NP_001157824.1 |
| RefSeq Size | 1802 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 58859 |
| Gene Summary | Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta (PubMed:16478991, PubMed:19855011, PubMed:20019329, PubMed:26486174, PubMed:26711913, PubMed:28508064). In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (PubMed:26690653, PubMed:26711913, PubMed:26220971, PubMed:26178373). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction (PubMed:17324935). Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling (PubMed:23636094).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) retains a segment in its 5' end which results in the use of an upstream start codon, compared to variant 1. The resulting protein (isoform 2) has a longer N-terminus when it is compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR207074 | Efemp2 (Myc-DDK-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2 |
CNY 3,656.00 |
|
| MR207074L3 | Lenti ORF clone of Efemp2 (Myc-DDK-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2 |
CNY 6,650.00 |
|
| MR207074L4 | Lenti ORF clone of Efemp2 (mGFP-tagged) - Mouse epidermal growth factor-containing fibulin-like extracellular matrix protein 2 (Efemp2), transcript variant 2 |
CNY 6,650.00 |
