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Klhl18 (BC025563) Mouse Tagged ORF Clone
CAT＃: MR206865
- TrueORF®
Klhl18 (Myc-DDK-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893)
ORF Plasmid: tGFP
"BC025563" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A930041K15, MGC36415 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206865 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGTGCTGTGTTGTACGATGCAGCCAACAGCTTCATCCACCAGCACTTTGTAGAGGTGTCTCTGT CCGAAGAGTTCCTGGCCCTGCCCTTGGAAGACGTGCTTGAGCTGGTGTCCCGGGATGAGCTGAATGTGAA GTCAGAGGAGCAGGTTTTTGAAGCTGCATTGGCCTGGGTCAGGTATGACCGGGAGCAGAGGGGACCATGC CTGCCCGAGCTGCTGTCCAATATCCGCCTGCCTCTTTGCCGGCCCCAGTTCTTATCAGATCGAGTGCAGC AAGATGACCTGGTACGGTGCTGTCACAAATGCAGGGACCTGGTCGATGAAGCAAAGGACTATCATCTGAT GCCAGAGCGCCGGCCCCACCTGCCAGCTTTCAGGACTCGGCCCCGATGCTGCACGTCCATCGCTGGGCTC ATCTACGCTGTGGGGGGCCTCAACTCAGCAGCAAATTTTTATGCAGGTGATTCCCTGAATGTGGTGGAAG TGTTCGACCCTATCGCCAATCGCTGGGAAAAGTGCCATCCCATGACAACAGCCCGAAGCCGTGTGGGTGT GGCTGTGGTGAACGGGCTCCTCTATGCTATCGGGGGATATGATGGTCAGTTGCGGCTGAGCACCGTGGAG GCCTACAATCCTGAGACGGACACATGGACCCGAGTGGGGAGCATGAATAGCAAGCGAAGTGCCATGGGGA CAGTCGTGCTGGATGGACAGATCTACGTGTGTGGAGGCTATGACGGCAACTCCTCCCTCAACTCTGTGGA GACCTACTCACCTGAGACGGACAAGTGGACAGTGGTGACTCCGATGAGCTCAAACCGGAGTGCTGCTGGG GTGACAGTCTTTGAGGGCAGGATATATGTGTCAGGAGGCCACGATGGCTTGCAGATCTTCAGCAGTGTGG AACACTACAACCACCACACGGCCACCTGGCACCCGGCAGCCAGCATGCTCAACAAGCGCTGCCGACACGG AGCCGCCTCCCTGGGAAGCAAGATGTTTGTCTGTGGGGGCTATGATGGCTCTGGCTTCCTCAGTATTGCT GAGATGTACAGCTCTGTGGCAGACCAGTGGTGTCTCATAGTACCCATGCACACACGCCGGAGCCGGGTCT CCCTCGTGGCCAGCTGTGGGCGCCTCTATGCAGTGGGAGGTTACGATGGACAGTCAAACCTAAGCTCAGT GGAGATGTACGACCCAGAGACGGACCGCTGGACATTTATGGCCCCCATGGCATGCCACGAGGGGGGGGTT GGTGTGGGCTGCATCCCTCTTCTCACCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206865 protein sequence
Red=Cloning site Green=Tags(s) MMCAVLYDAANSFIHQHFVEVSLSEEFLALPLEDVLELVSRDELNVKSEEQVFEAALAWVRYDREQRGPC LPELLSNIRLPLCRPQFLSDRVQQDDLVRCCHKCRDLVDEAKDYHLMPERRPHLPAFRTRPRCCTSIAGL IYAVGGLNSAANFYAGDSLNVVEVFDPIANRWEKCHPMTTARSRVGVAVVNGLLYAIGGYDGQLRLSTVE AYNPETDTWTRVGSMNSKRSAMGTVVLDGQIYVCGGYDGNSSLNSVETYSPETDKWTVVTPMSSNRSAAG VTVFEGRIYVSGGHDGLQIFSSVEHYNHHTATWHPAASMLNKRCRHGAASLGSKMFVCGGYDGSGFLSIA EMYSSVADQWCLIVPMHTRRSRVSLVASCGRLYAVGGYDGQSNLSSVEMYDPETDRWTFMAPMACHEGGV GVGCIPLLTI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC025563 |
| ORF Size | 1290 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025563, AAH25563 |
| RefSeq Size | 4559 bp |
| RefSeq ORF | 1292 bp |
| Locus ID | 270201 |
| MW | 47.5 kDa |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis (By similarity). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (By similarity). Regulates light- and dark-dependent alpha-transducin localization changes in rod photoreceptors through UNC119 ubiquitination and degradation (PubMed:31696965). Preferentially ubiquitinates the unphosphorylated form of UNC119 over the phosphorylated form (PubMed:31696965). In the presence of UNC119, under dark-adapted conditions alpha-transducin mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light (PubMed:31696965).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218672 | Klhl18 (untagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893), (10ug) |
CNY 6,940.00 |
|
| MG206865 | Klhl18 (tGFP-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893) |
CNY 2,760.00 |
|
| MR206865L3 | Lenti ORF clone of Klhl18 (Myc-DDK-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893) |
CNY 4,370.00 |
|
| MR206865L4 | Lenti ORF clone of Klhl18 (mGFP-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893) |
CNY 4,370.00 |
