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Pnpla2 (BC019188) Mouse Tagged ORF Clone
CAT＃: MR206855
- TrueORF®
Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544)
ORF Plasmid: tGFP
"BC019188" in other vectors (4)
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CNY 5,856.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TTS-2.2, Atgl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206855 representing BC019188
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCATGCCTGCCAAGGTGAAGCAGGTGCCAACATTATTGAGGTGTCCAAGGAGGCCCGGAAGCGGT TCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCATCCGTGGCTGTCTACTAAAGACCCTGCC TGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTCCCTGACTCGTGTTTCAGACGGAGAGAAC GTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAGGCCAATGTCTGCAGCACATTTATCCCGG TGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCTATGTGGATGGCGGCATTTCAGACAACTT GCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATTCTCAGGCGAGAGTGACATCTGCCCTCAG GACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACCAGCATCCAGTTCAACCTTCGCAATCTCT ACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCCTCCGAGAGATGTGCAAACAGGGCTACAG AGATGGACTTCGATTCCTTAGGAGGAATGGCCTACTGAACCAACCCAACCCTTTGCTGGCACTGCCCCCA GTTGTCCCCCAGGAAGAGGATGCAGAGGAAGCTGCTGTGGTGGAGGAGAGGGCTGGAGAGGAGGATCAAT TGCAGCCTTATAGAAAAGATCGAATTCTAGAGCACCTGCCTGCCAGACTCAATGAGGCCCTGCTGGAGGC CTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACATGCTACCAGTGCGCCTGGCAACGGCCATG ATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTCACCATCCGCTTGTTGGAGTGGCTGCCTG ATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTAGCATCTGCCAGTATCTGGTGATGAGGGC CAAGAGGAAATTGGGTGACCATCTGCCTTCCAGACTGTCTGAGCAGGTGGAACTGCGACGTGCCCAGTCT CTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCCCTACCCAACTGGGTACGAAACAACCTCT CACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTCAGCTACTGCTGGGTCTCTTCTGCACCAA TGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGCCAGCCCCACTGCCGCAGATCCTGCCACC CCACAGGATCCACCTGGCCTCCCGCCTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206855 representing BC019188
Red=Cloning site Green=Tags(s) MSHACQGEAGANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGEN VIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQ DSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPP VVPQEEDAEEAAVVEERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAM MVPYTLPLESAVSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGDHLPSRLSEQVELRRAQS LPSVPLSCATYSEALPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPAT PQDPPGLPPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC019188 |
| ORF Size | 1290 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC019188.1 |
| RefSeq Size | 2064 bp |
| RefSeq ORF | 1292 bp |
| Locus ID | 66853 |
| MW | 75.7 kDa |
| Gene Summary | Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218367 | Pnpla2 (untagged) - Mouse patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544), (10ug) |
CNY 6,940.00 |
|
| MG206855 | Pnpla2 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544) |
CNY 7,456.00 |
|
| MR206855L3 | Lenti ORF clone of Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544) |
CNY 6,180.00 |
|
| MR206855L4 | Lenti ORF clone of Pnpla2 (mGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (cDNA clone MGC:29206 IMAGE:5027544) |
CNY 6,180.00 |
