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Fbxo5 (NM_025995) Mouse Tagged ORF Clone
CAT＃: MR206701
- TrueORF®
Fbxo5 (Myc-DDK-tagged) - Mouse F-box protein 5 (Fbxo5)
ORF Plasmid: tGFP
"NM_025995" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2510044I10Rik; C85305; Emi1; Fbxo31 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGGCGCACCTGCAGTGACCTGCGCCGGCCGTCCTCCTGTCCCTGCCGTCTTGGCGCGCGGACTA CCGTGGACGGTTGTAAAGAAGAAAGCCCTGTTCTTTCTGTTACAATGAAGTGTTTTAATTGCAACCCTGA TCTTTCCGAGCTTGAAGTGGTGAAGCCTGAGGACAGTGGGATAGAAGCTTCCTACAGTCCCGTGTGTTTG GAACCTTCCTGTAATGACTGTGTTAGAAACCATGAGAGGTTGTCTTTCATCGACTCACCAATTGTGGGAC ATGATAACAAGGAAAATCAACGCGTACAAAACACACTAGATAGTTCAAACGAAACAGAAGAGCTAGAGGC CAGTAGACTGTATGAGGACAGTGGCTACTCATCTTTCACACAAAGTGACCGTGACGATGGCATCCTTATC CTGGAGAATTTCAGAAACAGTCCCCAGGCCCGTCTGCTGCCATCACAGAGCCCGGACCAGCATCCCAACA AAACCTTGCTGCCTGTCCTGCATTTTGAAAGAGTGGTTTGCTCAACACTAAAAAAGAATGGCAAGCGAAA CCCTAAAGTGGATCGAGAAATGCTGAAGGAAGTTATTGCCAGCGGGAACTTTAGACTGCAAAATATAATT GGCAAGAAAATGGGCCTGGAGCACCTAGACATCCTGGCTGAGCTCTCCCGCAGGGGATTCGTGCACCTGT TGGCTAACATTTTGACTAAGCTCAGCGGCATGGACTTAGTAAATCTGTCTAAAGTGAGCAGAATTTGGAA GAAGATACTGGAAAACAATAAGGGGGCGTTCCAGCTCTACAGCAAAACCATGCAGCGAGTCATTGAAAGC AGTAAGTTGTCACTACATGCTACAACGAGAGGATATGTTGTGGGCAGAGCTGCACTAACTTGTGTTCAAA AGTCATCGACCTGGGCACCTCCCAAAAAAGATGTTCAAATCAAGTCCTCCAGTCAGCGTGGTCAGAGAGT TTCTACCTACAGCCGGCACAATGAGTTCGTGGAGGTGGCAAAGACATTGAAGAACAACGAAAGCCTCAAA GCCTGTGTTCGCTGTAATTTCCCTGCAAAATATGACCACTATTTAGAGCGAGCAGTCTGCAAACGGGAAA GCTGTCAATTTGAATATTGTACAAAGTGTCTGTGTGCTTACCATAACAACAAAGACTGTTTGAATGGCAA GATCCTAAAAGCCAGCTGTAAAGTGGGTCCTTTGCCTGGAACTAAAAAGAGTAAAAAGAACTTACAAAGA TTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR206701 protein sequence
Red=Cloning site Green=Tags(s) MSRRTCSDLRRPSSCPCRLGARTTVDGCKEESPVLSVTMKCFNCNPDLSELEVVKPEDSGIEASYSPVCL EPSCNDCVRNHERLSFIDSPIVGHDNKENQRVQNTLDSSNETEELEASRLYEDSGYSSFTQSDRDDGILI LENFRNSPQARLLPSQSPDQHPNKTLLPVLHFERVVCSTLKKNGKRNPKVDREMLKEVIASGNFRLQNII GKKMGLEHLDILAELSRRGFVHLLANILTKLSGMDLVNLSKVSRIWKKILENNKGAFQLYSKTMQRVIES SKLSLHATTRGYVVGRAALTCVQKSSTWAPPKKDVQIKSSSQRGQRVSTYSRHNEFVEVAKTLKNNESLK ACVRCNFPAKYDHYLERAVCKRESCQFEYCTKCLCAYHNNKDCLNGKILKASCKVGPLPGTKKSKKNLQR L SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025995 |
| ORF Size | 1266 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025995.2, NP_080271.2 |
| RefSeq Size | 1820 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 67141 |
| UniProt ID | Q7TSG3 |
| MW | 47.5 kDa |
| Gene Summary | Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:17190794, PubMed:15526037, PubMed:16809773). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:16809773). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box co-receptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (By similarity). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (PubMed:15526037). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (PubMed:17190794). Promotes migration and osteogenic differentiation of mesenchymal stem cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205910 | Fbxo5 (untagged) - Mouse F-box protein 5 (Fbxo5), (10ug) |
CNY 2,000.00 |
|
| MG206701 | Fbxo5 (tGFP-tagged) - Mouse F-box protein 5 (Fbxo5) |
CNY 3,710.00 |
|
| MR206701L3 | Lenti ORF clone of Fbxo5 (Myc-DDK-tagged) - Mouse F-box protein 5 (Fbxo5) |
CNY 5,230.00 |
|
| MR206701L4 | Lenti ORF clone of Fbxo5 (mGFP-tagged) - Mouse F-box protein 5 (Fbxo5) |
CNY 5,230.00 |
