Wsb1 (NM_001042565) Mouse Tagged ORF Clone
CAT#: MR206694
- TrueORF®
Wsb1 (Myc-DDK-tagged) - Mouse WD repeat and SOCS box-containing 1 (Wsb1), transcript variant 2
"NM_001042565" in other vectors (3)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110056B13Rik; 2700038M07Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206694 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTTTCCCCCGAGGGTTAACGAGAAAGAGATCGTGAGATCACGTACTATAGGGGAACTCTTGG CTCCAGCAGCTCCTTTTGACAAGAAATGTGGTGGTGAGAACTGGACGGTTGCTTTTGCTCCTGATGGTTC CTACTTTGCGTGGTCGCAAGGATATCGCATAGTGAAGCTTGTCCCGTGGTCCCAGTGCCGTAAGAACTTT CTTCTGCATGGTTCCAAAAATGTTACCAATTCAAGCTGTCTAAAATTGGCAAGACAAAACAGTAATGGTG GTCAGAAAAACAAGCCTCCTGAGCACGTTATAGACTGTGGAGACATAGTCTGGAGTCTTGCTTTTGGGTC TTCAGTTCCAGAAAAACAGAGTCGTTGCGTTAATATAGAATGGCATCGGTTCCGATTTGGACAGGATCAG CTACTCCTTGCCACAGGATTAAACAATGGTCGCATCAAAATCTGGGATGTATATACAGGAAAACTCCTCC TTAATTTGGTAGACCACATTGAAATGGTTAGAGATTTAACTTTTGCTCCAGATGGGAGCTTACTCCTTGT ATCAGCTTCAAGAGACAAAACTCTAAGAGTGTGGGACCTGAAAGATGATGGAAACATGGTGAAAGTATTG CGGGCACATCAGAATTGGGTGTACAGTTGTGCATTCTCTCCCGACTGTTCTATGCTGTGTTCAGTTGGCG CCAGTAAAGCAGTTTTCCTTTGGAATATGGATAAATACACCATGATTAGGAAGCTGGAAGGTCATCACCA TGATGTTGTAGCTTGTGACTTTTCTCCTGATGGAGCATTGCTAGCTACTGCATCCTATGACACTCGTGTG TATGCCTGGGATCCACACAATGGAGACCTTCTGATGGAGTTTGGGCACCTGTTTCCCTCGCCCACTCCAA TATTTGCTGGAGGAGCAAATGACCGATGGGTGAGAGCTGTGTCTTTCAGTCATGATGGACTGCATGTTGC CAGCCTTGCTGATGATAAAATGGTGAGGTTCTGGAGAATCGATGAGGATTGTCCGGTACAAGTTGCACCT TTGAGCAATGGTCTTTGCTGTGCCTTTTCTACTGATGGCAGTGTTTTAGCTGCTGGGACACATGATGGAA GTGTGTATTTTTGGGCCACTCCAAGGCGAGTCCCTAGCCTTCAACATATATGTCGCATGTCAATCCGAAG AGTGATGTCCACCCAAGAAGTCCAAAAACTGCCTGTTCCTTCCAAAATATTGGCGTTTCTCTCCTACCGC GGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206694 protein sequence
Red=Cloning site Green=Tags(s) MASFPPRVNEKEIVRSRTIGELLAPAAPFDKKCGGENWTVAFAPDGSYFAWSQGYRIVKLVPWSQCRKNF LLHGSKNVTNSSCLKLARQNSNGGQKNKPPEHVIDCGDIVWSLAFGSSVPEKQSRCVNIEWHRFRFGQDQ LLLATGLNNGRIKIWDVYTGKLLLNLVDHIEMVRDLTFAPDGSLLLVSASRDKTLRVWDLKDDGNMVKVL RAHQNWVYSCAFSPDCSMLCSVGASKAVFLWNMDKYTMIRKLEGHHHDVVACDFSPDGALLATASYDTRV YAWDPHNGDLLMEFGHLFPSPTPIFAGGANDRWVRAVSFSHDGLHVASLADDKMVRFWRIDEDCPVQVAP LSNGLCCAFSTDGSVLAAGTHDGSVYFWATPRRVPSLQHICRMSIRRVMSTQEVQKLPVPSKILAFLSYR G TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001042565 |
ORF Size | 1266 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2346 bp |
RefSeq ORF | 540 bp |
Locus ID | 78889 |
MW | 47.1 kDa |
Gene Summary | Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes type II iodothyronine deiodinase/DIO2. Confers constitutive instability to HIPK2 through proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207777 | Wsb1 (untagged) - Mouse WD repeat and SOCS box-containing 1 (Wsb1), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MR206694L3 | Lenti ORF clone of Wsb1 (Myc-DDK-tagged) - Mouse WD repeat and SOCS box-containing 1 (Wsb1), transcript variant 2 |
CNY 2,950.00 |
|
MR206694L4 | Lenti ORF clone of Wsb1 (mGFP-tagged) - Mouse WD repeat and SOCS box-containing 1 (Wsb1), transcript variant 2 |
CNY 2,950.00 |