Sgms1 (NM_144792) Mouse Tagged ORF Clone
CAT#: MR206639
- TrueORF®
Sgms1 (Myc-DDK-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2
ORF Plasmid: tGFP
"NM_144792" in other vectors (4)
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CNY 5,488.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9530058O11Rik; AI841905; C80702; Mob; Sms1; Sor1; Tmem23 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206639 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCTGCCAGGACCATGAAGGAAGTGGTTTACTGGTCACCCAAGAAGGTGGCAGACTGGCTGCTGG AGAATGCTATGCCAGAATACTGTGAGCCTCTGGAGCACTTCACAGGCCAGGACTTAATCAACCTAACCCA AGAGGATTTCAAAAAACCCCCACTGTACCGAGTCTCCTCTGACAATGGGCAGCGACTCTTAGACATGATA GAGACCCTGAAGATGGAGCACCATATGGAAGCACACAAGAATGGCCACGCCAACGGACACCTCAGCATTG GCGTTGACATTCCCAACCCCGATGGCAGCTTCAGCATCAAGACTAAACCCAACGGAATGCCAAATGGGTT TAGGAAAGAGATGATCAAGATCCCCATGCCAGAACCGGAGCGCTCCCAGTATCCCATGGAGTGGGGCAAG ACTTTCCTGGCCTTTCTTTATGCACTTTCCTGTTTTGTTCTCACTACAGTGATGATCTCGGTCGTCCATG AACGAGTACCTCCTAAGGAGGTGCAGCCTCCACTACCGGACACGTTTTTTGACCATTTTAACCGGGTGCA GTGGGCGTTTTCTATTTGCGAAATTAACGGCATGATCCTTGTAGGACTCTGGCTATTTCAGTGGCTGCTC TTAAAATACAAGTCTATTATTAGCAGAAGATTTTTCTGCATAGTTGGCACGCTGTACCTGTATCGGTGTA TTACAATGTATGTAACTACACTCCCAGTACCTGGCATGCATTTCAACTGTTCTCCGAAGCTCTTTGGAGA CTGGGAAGCTCAAGTGCGGAGAATAATGAAGCTCATTGCTGGAGGTGGCTTATCCATCACAGGCTCGCAC AACATGTGTGGCGACTATCTGTACAGTGGCCACACGGTCATGCTAACGCTCACCTACCTATTTATCAAAG AGTATTCTCCTCGGCGGCTCTGGTGGTACCACTGGATTTGCTGGCTCCTCAGCGTCGTTGGAATCTTCTG TATTCTCTTAGCGCATGACCACTACACTGTGGACGTGGTGGTGGCCTACTACATCACCACAAGACTCTTC TGGTGGTATCACACGATGGCCAATCAGCAAGTGCTTAAGGAAGCCTCCCAGATGAACCTCCTGGCCAGGG TGTGGTGGTACAGGCCATTTCAGTACTTTGAAAAGAATGTCCAAGGAATTGTACCTCGATCTTACCATTG GCCCTTCCCCTGGCCGGTAGTCCACCTTAGTAGGCAAGTTAAATATAGCCGGCTGGTAAACGACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206639 protein sequence
Red=Cloning site Green=Tags(s) MLSARTMKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLYRVSSDNGQRLLDMI ETLKMEHHMEAHKNGHANGHLSIGVDIPNPDGSFSIKTKPNGMPNGFRKEMIKIPMPEPERSQYPMEWGK TFLAFLYALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLFQWLL LKYKSIISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQVRRIMKLIAGGGLSITGSH NMCGDYLYSGHTVMLTLTYLFIKEYSPRRLWWYHWICWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLF WWYHTMANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVVHLSRQVKYSRLVNDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144792 |
ORF Size | 1260 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144792.5 |
RefSeq Size | 3624 bp |
RefSeq ORF | 1260 bp |
Locus ID | 208449 |
UniProt ID | Q8VCQ6 |
MW | 49.3 kDa |
Gene Summary | Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types (By similarity). Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Visualizing intra-Golgi localization and transport by side-averaging Golgi ministacks
,null,
The Journal of Cell Biology
,PubMed ID 35467701
[Sgms1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202951 | Sgms1 (untagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
MG206639 | Sgms1 (tGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1) |
CNY 7,088.00 |
|
MR206639L3 | Lenti ORF clone of Sgms1 (Myc-DDK-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 |
CNY 4,750.00 |
|
MR206639L4 | Lenti ORF clone of Sgms1 (mGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 |
CNY 4,750.00 |