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Chst1 (NM_023850) Mouse Tagged ORF Clone
CAT＃: MR206464
- TrueORF®
Chst1 (Myc-DDK-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1)
ORF Plasmid: tGFP
"NM_023850" in other vectors (4)
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CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610008E20Rik; AW125896; C6ST; GST-1; Gst1; KSGAL6ST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206464 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATGTTCTTGGAAGGCTGTCCTCCTCCTCGCCCTGGCCTCCATTGCCATCCAGTACACAGCCATCC GCACTTTCACCGCCAAGTCTTTCCACACTTGCCCTGGGCTGACAGATACTGGCTTGGCAGAGCGGTTGTG TGAGGAGGGCCCTACCTTCTCCTACAACCTCTCCAGGAAGACCCACGTCCTGATCCTGGCCACCACACGC AGTGGCTCTTCCTTTGTGGGCCAGCTCTTCAACCAGCACATGGATGTCTTCTACCTGTTCGAGCCGCTCT ATCACGTCCAAAACACGCTCATCCCCCGGTTCACCCAGGGCAAGAGCCCCGCAGATCGCAGGGTCATGCT AGGTGCCAGCCGGGACCTGCTGAGGAGCCTTTATGATTGTGATCTCTACTTTCTGGAGAACTACATCAAG CCACCTCCAGTCAACCACACCACCAACAGGGTCTTCCGCAGAGGGGCCAGCAGGGTCCTTTGTTCCCGTC CAGTGTGTGACCCTCCAGGGTCCTCTGACCTGATCCTGGAGGAGGGGGACTGTGTGCGCATGTGTGGCTT GCTGAACCTGACCTTGGCAGCCGAGGCTTGTCGTGAGCGCAGTCATGTGGCCATCAAGACTGTGCGGGTG CCCGAGGTGAATGACTTGCGGGCTCTAGTGGAAGACCCCAGGCTGAACCTCAAGGTCATCCAGCTGGTGC GAGACCCTCGTGGCATCTTGGCTTCTCGCAGTGAGACCTTCCGGGACACCTACCGGCTCTGGCGGCTTTG GTACGGCACAGGGAGGAAGCCCTACAACCTGGATGTGACACAGCTGACCACTGTGTGTGAGGATTTCTCT AGCTCTGTGTCTACTGGCCTCATGCGGCCCTCCTGGCTCAAGGGCAAGTACATGCTGGTACGTTATGAGG ACCTGGCCAGAAATCCAATGAAGAAGACAGAAGAGATCTACGAATTCCTGGGTATCCCCCTGGACAGTCA CGTGGCACACTGGATCCAGAACAATACGAGGGGCGACCCCACTTTGGGCAAGCACAAATACAGCACAGTG CGCAACTCTGCAGCCACAGCTGAGAAGTGGCGCTTCCGCCTCTCCTATGACATCGTGGCCTTTGCCCAGA ATGCCTGCCAGCAGGTGTTGGCTCAGCTGGGCTACAAGATGGCCAACTCGGAGGAGGAACTGAAGAATCC GGCCATCAGCCTAGTGGAGGAGCGTGACTTCCGCCCTTTTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206464 protein sequence
Red=Cloning site Green=Tags(s) MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLTDTGLAERLCEEGPTFSYNLSRKTHVLILATTR SGSSFVGQLFNQHMDVFYLFEPLYHVQNTLIPRFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIK PPPVNHTTNRVFRRGASRVLCSRPVCDPPGSSDLILEEGDCVRMCGLLNLTLAAEACRERSHVAIKTVRV PEVNDLRALVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVCEDFS SSVSTGLMRPSWLKGKYMLVRYEDLARNPMKKTEEIYEFLGIPLDSHVAHWIQNNTRGDPTLGKHKYSTV RNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKMANSEEELKNPAISLVEERDFRPFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023850 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023850.1, NP_076339.1 |
| RefSeq Size | 2682 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 76969 |
| UniProt ID | Q9EQC0 |
| MW | 46.9 kDa |
| Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
KSGal6ST Is Essential for the 6-Sulfation of Galactose within Keratan Sulfate in Early Postnatal Brain
,Hoshino, H;Foyez, T;Ohtake-Niimi, S;Takeda-Uchimura, Y;Michikawa, M;Kadomatsu, K;Uchimura, K;,
J. Histochem. Cytochem.
,PubMed ID 24152993
[Chst1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208075 | Chst1 (untagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1), (10ug) |
CNY 3,990.00 |
|
| MG206464 | Chst1 (tGFP-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 3,140.00 |
|
| MR206464L3 | Lenti ORF clone of Chst1 (Myc-DDK-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 4,750.00 |
|
| MR206464L4 | Lenti ORF clone of Chst1 (mGFP-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 4,750.00 |
