Chst1 (NM_023850) Mouse Untagged Clone

CAT#: MC208075

Chst1 (untagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1), (10ug)



  "NM_023850" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2610008E20Rik; AW125896; C6ST; GST-1; Gst1; KSGAL6ST
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208075 representing NM_023850
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAATGTTCTTGGAAGGCTGTCCTCCTCCTCGCCCTGGCCTCCATTGCCATCCAGTACACAGCCATCC
GCACTTTCACCGCCAAGTCTTTCCACACTTGCCCTGGGCTGACAGATACTGGCTTGGCAGAGCGGCTGTG
TGAGGAGGGCCCTACCTTCTCCTACAACCTCTCCAGGAAGACCCACGTCCTGATCCTGGCCACCACACGC
AGTGGCTCTTCCTTTGTGGGCCAGCTCTTCAACCAGCACATGGATGTCTTCTACCTGTTCGAGCCGCTCT
ATCACGTCCAAAACACGCTCATCCCCCGGTTCACCCAGGGCAAGAGCCCCGCAGATCGCAGGGTCATGCT
AGGTGCCAGCCGGGACCTGCTGAGGAGCCTTTATGATTGTGATCTCTACTTTCTGGAGAACTACATCAAG
CCACCTCCAGTCAACCACACCACCAACAGGGTCTTCCGCAGAGGGGCCAGCAGGGTCCTTTGCTCCCGTC
CAGTGTGTGACCCTCCAGGGTCCTCTGACCTGATCCTGGAGGAGGGGGACTGTGTGCGCATGTGTGGCTT
GCTGAACCTGACCTTGGCAGCCGAGGCTTGTCGTGAGCGCAGTCATGTGGCCATCAAGACTGTGCGGGTG
CCCGAGGTGAATGACTTGCGGGCTCTAGTGGAAGACCCCAGGCTGAACCTCAAGGTCATCCAGCTGGTGC
GAGACCCTCGTGGCATCTTGGCTTCTCGCAGTGAGACCTTCCGGGACACCTACCGGCTCTGGCGGCTTTG
GTACGGCACAGGGAGGAAGCCCTACAACCTGGATGTGACACAGCTGACCACTGTGTGTGAGGATTTCTCT
AGCTCTGTGTCTACTGGCCTCATGCGGCCCTCCTGGCTCAAGGGCAAGTACATGCTGGTACGTTATGAGG
ACCTGGCCAGAAATCCAATGAAGAAGACAGAAGAGATCTACGAATTCCTGGGTATCCCCCTGGACAGTCA
CGTGGCACACTGGATCCAGAACAATACGAGGGGCGACCCCACTTTGGGCAAGCACAAATACAGCACAGTG
CGCAACTCTGCAGCCACAGCTGAGAAGTGGCGCTTCCGCCTCTCCTATGACATCGTGGCCTTTGCCCAGA
ATGCCTGCCAGCAGGTGTTGGCTCAGCTGGGCTACAAGATGGCCAACTCAGAGGAGGAACTGAAGAATCC
GGCCATCAGCCTAGTGGAGGAGCGTGACTTCCGCCCTTTTTTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_023850
Insert Size 1236 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_023850.2, NP_076339.1
RefSeq Size 2682 bp
RefSeq ORF 1236 bp
Locus ID 76969
UniProt ID Q9EQC0
Gene Summary Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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