United States
Germany
Japan
United Kingdom
China
Chst1 (NM_023850) Mouse Untagged Clone
CAT＃: MC208075
Chst1 (untagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610008E20Rik; AW125896; C6ST; GST-1; Gst1; KSGAL6ST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208075 representing NM_023850
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATGTTCTTGGAAGGCTGTCCTCCTCCTCGCCCTGGCCTCCATTGCCATCCAGTACACAGCCATCC GCACTTTCACCGCCAAGTCTTTCCACACTTGCCCTGGGCTGACAGATACTGGCTTGGCAGAGCGGCTGTG TGAGGAGGGCCCTACCTTCTCCTACAACCTCTCCAGGAAGACCCACGTCCTGATCCTGGCCACCACACGC AGTGGCTCTTCCTTTGTGGGCCAGCTCTTCAACCAGCACATGGATGTCTTCTACCTGTTCGAGCCGCTCT ATCACGTCCAAAACACGCTCATCCCCCGGTTCACCCAGGGCAAGAGCCCCGCAGATCGCAGGGTCATGCT AGGTGCCAGCCGGGACCTGCTGAGGAGCCTTTATGATTGTGATCTCTACTTTCTGGAGAACTACATCAAG CCACCTCCAGTCAACCACACCACCAACAGGGTCTTCCGCAGAGGGGCCAGCAGGGTCCTTTGCTCCCGTC CAGTGTGTGACCCTCCAGGGTCCTCTGACCTGATCCTGGAGGAGGGGGACTGTGTGCGCATGTGTGGCTT GCTGAACCTGACCTTGGCAGCCGAGGCTTGTCGTGAGCGCAGTCATGTGGCCATCAAGACTGTGCGGGTG CCCGAGGTGAATGACTTGCGGGCTCTAGTGGAAGACCCCAGGCTGAACCTCAAGGTCATCCAGCTGGTGC GAGACCCTCGTGGCATCTTGGCTTCTCGCAGTGAGACCTTCCGGGACACCTACCGGCTCTGGCGGCTTTG GTACGGCACAGGGAGGAAGCCCTACAACCTGGATGTGACACAGCTGACCACTGTGTGTGAGGATTTCTCT AGCTCTGTGTCTACTGGCCTCATGCGGCCCTCCTGGCTCAAGGGCAAGTACATGCTGGTACGTTATGAGG ACCTGGCCAGAAATCCAATGAAGAAGACAGAAGAGATCTACGAATTCCTGGGTATCCCCCTGGACAGTCA CGTGGCACACTGGATCCAGAACAATACGAGGGGCGACCCCACTTTGGGCAAGCACAAATACAGCACAGTG CGCAACTCTGCAGCCACAGCTGAGAAGTGGCGCTTCCGCCTCTCCTATGACATCGTGGCCTTTGCCCAGA ATGCCTGCCAGCAGGTGTTGGCTCAGCTGGGCTACAAGATGGCCAACTCAGAGGAGGAACTGAAGAATCC GGCCATCAGCCTAGTGGAGGAGCGTGACTTCCGCCCTTTTTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_023850 |
| Insert Size | 1236 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023850.2, NP_076339.1 |
| RefSeq Size | 2682 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 76969 |
| UniProt ID | Q9EQC0 |
| Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206464 | Chst1 (tGFP-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 3,140.00 |
|
| MR206464 | Chst1 (Myc-DDK-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 3,656.00 |
|
| MR206464L3 | Lenti ORF clone of Chst1 (Myc-DDK-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 4,750.00 |
|
| MR206464L4 | Lenti ORF clone of Chst1 (mGFP-tagged) - Mouse carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (Chst1) |
CNY 4,750.00 |
