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Lox (NM_010728) Mouse Tagged ORF Clone
CAT＃: MR206463
- TrueORF®
Lox (Myc-DDK-tagged) - Mouse lysyl oxidase (Lox)
"NM_010728" in other vectors (3)
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CNY 3,656.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI893619; rrg; TSC-16; TSC-160 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206463 representing NM_010728
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTTCGCCTGGGCTGTGCTCCTTCTGGGGCCACTGCAGCTTTGTCCCCTTCTCCGCTGCGCCCCGC AGACCCCGCGCGAGCCGCCCGCCGCCCCTGGTGCCTGGCGCCAGACAATCCAATGGGAGAACAACGGGCA GGTGTTCAGTCTGCTGAGCCTCGGGGCGCAGTACCAGCCTCAGCGACGCCGCGACCCCAGTGCCACTGCC CGGAGACCCGACGGCGACGCAGCCTCGCAGCCGCGCACGCCCATTCTTCTGCTGCGTGACAACCGCACCG CCTCTACCCGTGCGAGGACGCCAAGCCCGTCTGGGGTCGCCGCGGGTCGTCCCCGGCCCGCCGCCCGCCA CTGGTTCCAAGCTGGTTTCTCGCCGTCGGGGGCTCGCGATGGAGCCTCACGGCGCGCGGCGAACCGGACT GCATCGCCACAGCCTCCGCAGCTCAGTAATCTGAGGCCACCCAGCCACATAGATCGCATGGTGGGCGACG ACCCCTACAATCCCTACAAGTACTCCGACGACAACCCCTATTATAACTACTATGACACGTATGAGAGGCC CCGGCCCGGGAGCAGGAACCGACCTGGATACGGCACCGGTTACTTCCAGTACGGTCTCCCGGACCTGGTG CCCGACCCCTACTACATCCAGGCTTCCACGTACGTCCAGAAGATGTCTATGTACAACCTGAGATGCGCTG CGGAAGAAAACTGCCTGGCCAGTTCAGCATATAGGGCGGATGTCAGAGACTATGACCACAGGGTACTGCT ACGATTTCCGCAAAGAGTGAAGAACCAAGGGACATCGGACTTCTTACCAAGCCGCCCTCGGTACTCCTGG GAGTGGCACAGCTGTCACCAACATTACCACAGCATGGACGAATTCAGCCACTATGACCTGCTTGATGCCA ACACACAGAGGAGAGTGGCTGAAGGCCACAAAGCAAGCTTCTGTCTGGAGGACACGTCCTGTGACTATGG GTACCACAGGCGCTTTGCGTGCACTGCACACACACAGGGATTGAGTCCTGGATGTTATGACACCTATGCG GCAGACATAGACTGCCAGTGGATTGATATTACAGATGTACAACCTGGAAACTACATTCTAAAGGTCAGTG TAAACCCCAGCTACCTGGTGCCTGAATCAGACTACACTAACAATGTTGTACGCTGTGACATTCGCTACAC AGGACATCATGCCTATGCCTCAGGCTGCACAATTTCACCGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206463 representing NM_010728
Red=Cloning site Green=Tags(s) MRFAWAVLLLGPLQLCPLLRCAPQTPREPPAAPGAWRQTIQWENNGQVFSLLSLGAQYQPQRRRDPSATA RRPDGDAASQPRTPILLLRDNRTASTRARTPSPSGVAAGRPRPAARHWFQAGFSPSGARDGASRRAANRT ASPQPPQLSNLRPPSHIDRMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGSRNRPGYGTGYFQYGLPDLV PDPYYIQASTYVQKMSMYNLRCAAEENCLASSAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSW EWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYA ADIDCQWIDITDVQPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010728 |
| ORF Size | 1233 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010728.3 |
| RefSeq Size | 3604 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 16948 |
| UniProt ID | P28301 |
| MW | 47.2 kDa |
| Gene Summary | This gene encodes a precursor protein that belongs to the lysyl oxidase family of proteins. The secreted proprotein is proteolytically processed to an active mature peptide and a propeptide. This propeptide is thought to function in tumor suppression by inhibiting the Ras signaling pathway. The active enzyme plays a role in cross-linking of collagen and elastin and is essential for development of cardiovascular and respiratory systems, and development of skin and connective tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression
,Maller, O;Drain, AP;Barrett, AS;Borgquist, S;Ruffell, B;Zakharevich, I;Pham, TT;Gruosso, T;Kuasne, H;Lakins, JN;Acerbi, I;Barnes, JM;Nemkov, T;Chauhan, A;Gruenberg, J;Nasir, A;Bjarnadottir, O;Werb, Z;Kabos, P;Chen, YY;Hwang, ES;Park, M;Coussens, LM;Nelson, AC;Hansen, KC;Weaver, VM;,
Nature materials
,PubMed ID 33257795
[LOX]
|
|
Inflammation promotes tumor aggression by stimulating stromal cell-dependent collagen crosslinking and stromal stiffening
,Maller, O;Drain, AP;Barrett, AS;Borgquist, S;Ruffell, B;,
bioRxiv
[LOX]
|
|
Lysyl oxidase drives tumour progression by trapping EGF receptors at the cell surface
,Tang, H;Leung, L;Saturno, G;Viros, A;Smith, D;Di Leva, G;Morrison, E;Niculescu-Duvaz, D;Lopes, F;Johnson, L;Dhomen, N;Springer, C;Marais, R;,
Nat Commun
,PubMed ID 28416796
[LOX]
|
|
LOX Fails to Substitute for RANKL in Osteoclastogenesis
,Tsukasaki, M;Hamada, K;Okamoto, K;Nagashima, K;Terashima, A;Komatsu, N;Win, S;Okamura, T;Nitta, T;Yasuda, H;Penninger, JM;Takayanagi, H;,
J. Bone Miner. Res.
,PubMed ID 27606829
[Lox]
|
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208031 | Lox (untagged) - Mouse lysyl oxidase (Lox), (10ug) |
CNY 3,656.00 |
|
| MR206463L3 | Lenti ORF clone of Lox (Myc-DDK-tagged) - Mouse lysyl oxidase (Lox) |
CNY 5,230.00 |
|
| MR206463L4 | Lenti ORF clone of Lox (mGFP-tagged) - Mouse lysyl oxidase (Lox) |
CNY 6,056.00 |
