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Aup1 (BC016485) Mouse Tagged ORF Clone
CAT＃: MR206456
- TrueORF®
Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982)
ORF Plasmid: tGFP
"BC016485" in other vectors (4)
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CN¥ 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA589454 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206456 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCTCCCCCAGCTCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCTCCCGAGTGACGGCTTCC TGCTGCTCGCGCTACTGCTCTACGCGCCGGTCGGCCTCTGCCTCCTGGTCCTGCGACTCTTCCTCGGGCT CCACGTCTTCCTGGTCAGCTGCGCTCTGCCCGACAGCGTCCTCCGCAGGTTCGTGGTACGAACCATGTGT GCGGTCCTGGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGCGATCACCGCGTCAGGGTCCTTATTT CCAACCACGTAACACCTTTTGACCACAACATAGTCAACCTCCTCACCACCTGTAGCACCCCTCTACTCAA TAGTCCCCCCAGCTTTGTGTGTTGGTCTCGGGGCTTCATGGAGATGGATAGGCGGGTGGAGTTGGTGGAG TCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGCCCACACCTTTGCTGCTCTTCCCCGAGGAAGAGG CCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTCGTGGCCATTTTCTATTCAGGACGTGGTACAACC TCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCTCCTGGGTCTCAGAACTG CTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAGTAAGGTGGCTTCATCCCATTCGTCGACAGCTAG GGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACAGCTGGTGGCCAAAGAATTGGGCCAGATAGGGAC ACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAGCGACAAAGACACCCCAGATTACGCCCCCAGTCA GTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTTCTGATGTGCAGCTGACCACTCTCGCTCACAGAG TCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCATCCAGAGAGACCTGGCCAGGACTGGGTGTGTAGA CTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCTTTCATGCCTGAAGATGTCACTGAGGGATCTCAG TCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCTCGGGCCTGGCGACCCCTCAGCCCACAGCCCTAA CGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCTGCAGGAGCGCAAGCAGGCACTGTATGAATATGC AAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206456 protein sequence
Red=Cloning site Green=Tags(s) MEPPPAPGPERLFDSHRLPSDGFLLLALLLYAPVGLCLLVLRLFLGLHVFLVSCALPDSVLRRFVVRTMC AVLGLVARQEDSGLRDHRVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMDRRVELVE SLKKFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSEL LWSLFVPFTVYQVRWLHPIRRQLGEESEEFALRVQQLVAKELGQIGTRLTPADKAEHMKRQRHPRLRPQS VQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLARTGCVDLTITNLLEGAVAFMPEDVTEGSQ SPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQALYEYARRRFRERQAQEAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC016485 |
| ORF Size | 1230 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016485, AAH16485 |
| RefSeq Size | 1454 bp |
| RefSeq ORF | 1232 bp |
| Locus ID | 11993 |
| MW | 46.1 kDa |
| Gene Summary | The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218140 | Aup1 (untagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982), (10ug) |
CN¥ 6,940.00 |
|
| MG206456 | Aup1 (tGFP-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) |
CN¥ 2,660.00 |
|
| MR206456L3 | Lenti ORF clone of Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) |
CN¥ 4,280.00 |
|
| MR206456L4 | Lenti ORF clone of Aup1 (mGFP-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982) |
CN¥ 4,280.00 |
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