Cyth2 (NM_011181) Mouse Tagged ORF Clone
CAT#: MR206279
- TrueORF®
Cyth2 (Myc-DDK-tagged) - Mouse cytohesin 2 (Cyth2), transcript variant 1
ORF Plasmid: tGFP
"NM_011181" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ARNO; CLM2; Pscd2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206279 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGTGTCTACGAGCCCCCAGACTTGACTCCAGAGGAGCGCATGGAGTTGGAGAACATCCGGC GGCGCAAGCAGGAGCTGCTGGTGGAGATCCAGCGCCTGCGAGAAGAGCTAAGTGAAGCTATGAGCGAGGT GGAGGGTCTGGAGGCCAATGAGGGCAGTAAGACCTTGCAGCGGAACCGGAAGATGGCAATGGGCAGGAAG AAATTCAACATGGACCCCAAAAAGGGGATCCAATTCTTGGTGGAGCATGAACTTCTGCAGAACACGCCTG AGGAAATTGCCCGCTTCCTGTACAAGGGCGAGGGGCTGAACAAGACAGCCATTGGGGACTACCTAGGGGA AAGGGAAGAGCTGAACCTGTCTGTGCTCCATGCTTTTGTGGATCTCCATGAGTTCACCGACCTCAACCTG GTGCAGGCCCTCCGGCAATTCCTATGGAGCTTTCGCCTCCCTGGAGAGGCTCAGAAAATTGATCGAATGA TGGAGGCCTTTGCCCAGAGATACTGCTTATGCAACCCTGGGGTCTTCCAGTCTACAGACACCTGCTACGT GTTGTCCTTTGCCGTGATCATGCTGAACACCAGCCTTCACAATCCCAATGTCAGGGACAAGCCGGGCCTG GAGCGCTTTGTGGCCATGAACCGGGGCATCAACGAGGGTGGGGATCTGCCTGAGGATCTTCTCAGGAACC TCTACGACAGCATCCGGAACGAGCCCTTCAAAATCCCAGAGGATGATGGGAATGATCTCACCCACACCTT CTTCAACCCAGACCGAGAGGGCTGGCTCCTAAAGCTGGGGGGCCGGGTGAAGACTTGGAAGCGGCGCTGG TTCATCCTTACAGACAACTGCCTCTACTACTTTGAATACACTACGGACAAGGAGCCCCGAGGGATTATCC CCCTAGAGAACCTGAGCATCCGGGAGGTGGACGATCCTCGGAAGCCGAACTGCTTTGAGCTTTACATTCC CAACAATAAGGGACAGCTCATCAAAGCCTGCAAAACAGAGGCTGACGGCCGGGTAGTGGAGGGGAACCAC ATGGTGTACCGAATCTCAGCACCCACCCAGGAGGAGAAAGATGAGTGGATCAAATCCATCCAGGCTGCTG TAAGTGTGGACCCCTTCTATGAGATGTTGGCAGCAAGAAAGAAGCGAATTTCTGTGAAGAAGAAGCAAGA ACAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206279 protein sequence
Red=Cloning site Green=Tags(s) MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRK KFNMDPKKGIQFLVEHELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLSVLHAFVDLHEFTDLNL VQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGL ERFVAMNRGINEGGDLPEDLLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRW FILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNH MVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011181 |
ORF Size | 1200 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011181.1, NM_011181.2, NM_011181.3, NP_035311.1 |
RefSeq Size | 2527 bp |
RefSeq ORF | 1203 bp |
Locus ID | 19158 |
UniProt ID | P63034 |
MW | 46.5 kDa |
Gene Summary | Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (PubMed:18042453). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (By similarity). Involved in neurite growth (PubMed:25326380).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200196 | Cyth2 (untagged) - Mouse cytohesin 2 (Cyth2), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
MG206279 | Cyth2 (tGFP-tagged) - Mouse pleckstrin homology, Sec7 and coiled-coil domains 2 (Pscd2) |
CNY 3,140.00 |
|
MR206279L3 | Lenti ORF clone of Cyth2 (Myc-DDK-tagged) - Mouse cytohesin 2 (Cyth2), transcript variant 1 |
CNY 4,750.00 |
|
MR206279L4 | Lenti ORF clone of Cyth2 (mGFP-tagged) - Mouse cytohesin 2 (Cyth2), transcript variant 1 |
CNY 4,750.00 |