Rragc (NM_017475) Mouse Tagged ORF Clone
CAT#: MR206250
- TrueORF®
Rragc (Myc-DDK-tagged) - Mouse Ras-related GTP binding C (Rragc)
ORF Plasmid: tGFP
"NM_017475" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AU041672; Gtr2; RAGC; TIB929; YGR163W |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206250 representing NM_017475
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGCAGTACGGGGCGGAGGAGACGCCCCTGGCCGGCAGTTACGGCGCAGCCGACTCGTTCCCCA AGGACTTCGGCTACGGCGTGGAGGAGGAGGAAGAGGAGGCAGCGGCGGGTGGCGGCGGAGGCGCGGGGGC CGGCGGCGGCTGCGGCCCGGGCGGCGCTGACAGCTCCAAGCCGAGGATCCTGCTTATGGGGCTCCGGCGC AGCGGCAAATCCTCCATCCAGAAGGTGGTGTTTCATAAGATGTCACCCAATGAGACTCTCTTTTTGGAAA GTACCAACAAGATTTATAAAGATGACATTTCCAACAGCTCCTTTGTGAACTTCCAGATTTGGGATTTTCC TGGGCAGATGGACTTCTTTGATCCAACCTTTGACTACGAAATGATCTTCAGGGGCACAGGAGCATTGATA TATGTCATTGATGCACAGGACGACTACATGGAGGCTTTAACCCGACTTCACATCACTGTTTCTAAGGCCT ACAAAGTTAACCCAGACATGAATTTTGAGGTTTTTATTCACAAAGTTGATGGACTGTCTGATGATCACAA AATAGAAACACAGAGGGACATTCATCAAAGGGCCAATGATGACCTTGCAGATGCTGGGCTAGAAAAGCTC CATCTTAGCTTTTATTTGACTAGCATCTATGACCATTCAATATTTGAAGCCTTCAGTAAGGTGGTACAGA AACTCATTCCACAACTGCCCACTTTGGAAAACCTATTAAATATCTTTATATCAAATTCAGGTATTGAAAA AGCTTTTCTCTTTGATGTTGTCAGCAAAATCTACATTGCAACAGACAGCTCCCCTGTGGATATGCAGTCT TATGAACTTTGCTGTGACATGATTGATGTTGTAATTGATGTGTCTTGTATATATGGATTAAAGGAAGATG GAAGTGGAAGTGCTTATGACAAAGAATCGATGGCCATTATCAAGCTGAATAATACAACTGTCCTTTATTT AAAGGAAGTCACTAAATTTTTGGCACTGGTTTGCATTCTTAGGGAAGAGAGCTTTGAACGAAAAGGGTTG ATAGACTACAACTTCCACTGTTTCCGAAAAGCTATCCATGAGGTGTTTGAGGTGGGTGTGACTTCTCACA GAAGCTGCAGTCATCAGACCAGTGCTCCAAGTCTGAAAGCCTTGGCACATAACGGCACGCCTCGGAATGC CATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206250 representing NM_017475
Red=Cloning site Green=Tags(s) MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAGGGGGAGAGGGCGPGGADSSKPRILLMGLRR SGKSSIQKVVFHKMSPNETLFLESTNKIYKDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALI YVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKL HLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQS YELCCDMIDVVIDVSCIYGLKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGL IDYNFHCFRKAIHEVFEVGVTSHRSCSHQTSAPSLKALAHNGTPRNAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017475 |
ORF Size | 1194 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017475.2, NP_059503.2 |
RefSeq Size | 2621 bp |
RefSeq ORF | 1197 bp |
Locus ID | 54170 |
UniProt ID | Q99K70 |
MW | 44.1 kDa |
Gene Summary | Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209956 | Rragc (untagged) - Mouse Ras-related GTP binding C (Rragc), (10ug) |
CNY 3,990.00 |
|
MG206250 | Rragc (tGFP-tagged) - Mouse Ras-related GTP binding C (Rragc) |
CNY 3,140.00 |
|
MR206250L3 | Lenti ORF clone of Rragc (Myc-DDK-tagged) - Mouse Ras-related GTP binding C (Rragc) |
CNY 4,750.00 |
|
MR206250L4 | Lenti ORF clone of Rragc (mGFP-tagged) - Mouse Ras-related GTP binding C (Rragc) |
CNY 4,750.00 |