Terf2ip (BC017641) Mouse Tagged ORF Clone

CAT#: MR206145

  • TrueORF®

Terf2ip (Myc-DDK-tagged) - Mouse telomeric repeat binding factor 2, interacting protein (cDNA clone MGC:28971 IMAGE:4483320)

ORF Plasmid: DDK tGFP



  "BC017641" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

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经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Rap1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206145 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCGATGGATCTGGGTAAAGACCCCAATGGGCCCACTCACTCCTCCACTCTGTTCGTGAGAG
AAGACGGCAGCGCCATGTCGTTTTACGTGCGGCCCAGCTCGGCCAAGCGCCGGCTGTCGACGCTCATCCT
GCACGGCGGCGGCACCGTGTGTCGGGTGCAGGAACCCGGAGCCGTGCTTCTCGCCCAGCCCGGGGAGGCG
CTGGCCGAGGCTTCGGGCGACTTCATCTCCACGCAGTACATCCTAGACTGCGTGGATCGCAACGAGAAGC
TGGACCTGGAGGCCTATCGGCTGGGCCTGACGGAGCAGGCGTCCGATCCGAAGCCCGGGGCTTCCACCGA
GGGCTCCACGGAACCGGAGCCGCAGCCCCTGACCGGGCGCATCGCCTACACCGACGCGGAAGATGTGGCC
ATCCTGACCTACGTGAAGGAGAACGCCCGTTCGCCCAGCTCGGTCACAGGCAATGCCTTGTGGAAAGCGA
TGGAGAAGAGCTCGCTCACGCAGCACTCCTGGCAGTCGCTCAAGGACCGCTACCTCAAGCACCTACGGGG
TCAGGAGCACAAGTACCTGCTCGGGAACGCCCCGGTCAGCCCGTCCTCCCAGAAGCTCAAACGGAAGGCG
GAGCAGGACCCCGAGGCCGCGGATAGCGGAGAGCCACAGAACAAGAGAGCGCCAGACTTGCCTGAGGAGG
AGTGTGTGAAAGGAGAGATCAAGGAGAATGGAGAGGCAGACAACAAGCTGTTTGAGGAAGCCGCTCCGGA
GTTCGGAGAAGCCGTGGTGGATGAGAGCCCTGACTTTGAAATACATATAACGATGTGTGATGGTGATCCA
CCCACACCCGAGGAAGACTCAGAAACACAGCCAGACGAGGAGGAAGAAGAACCAAAAGTTTCTACGCAAG
AAGTGGGAACTGCCATTAAGGTGATCCGGCAGCTAATGGAGAAGTTCAACTTGGATCTATCAACAGTTAC
ACAGGCCTTGCTGAAGAACAGTGGTGAGCTGGAGGCCACGTCCTCCTTCTTAGAGTCGGGGCGGAGACCC
GACGGTTATCCCATTTGGTGCAGACAAGATGACTTAGATTTGCAGAAGGACGATGACGACACGAAAAATG
CACTGGTCAAAAAGTTTGGAGCTCAGAACGTTGCTCGGAGGATTGAATTCCGAAAGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206145 protein sequence
Red=Cloning site Green=Tags(s)

MAEAMDLGKDPNGPTHSSTLFVREDGSAMSFYVRPSSAKRRLSTLILHGGGTVCRVQEPGAVLLAQPGEA
LAEASGDFISTQYILDCVDRNEKLDLEAYRLGLTEQASDPKPGASTEGSTEPEPQPLTGRIAYTDAEDVA
ILTYVKENARSPSSVTGNALWKAMEKSSLTQHSWQSLKDRYLKHLRGQEHKYLLGNAPVSPSSQKLKRKA
EQDPEAADSGEPQNKRAPDLPEEECVKGEIKENGEADNKLFEEAAPEFGEAVVDESPDFEIHITMCDGDP
PTPEEDSETQPDEEEEEPKVSTQEVGTAIKVIRQLMEKFNLDLSTVTQALLKNSGELEATSSFLESGRRP
DGYPIWCRQDDLDLQKDDDDTKNALVKKFGAQNVARRIEFRKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC017641
ORF Size 1179 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC017641, AAH17641
RefSeq Size 2122 bp
RefSeq ORF 1181 bp
Locus ID 57321
MW 43.4 kDa
Gene Summary Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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