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Atg4b (NM_174874) Mouse Tagged ORF Clone
CAT＃: MR206129
- TrueORF®
Atg4b (Myc-DDK-tagged) - Mouse autophagy-related 4B (yeast) (Atg4b)
ORF Plasmid: tGFP
"NM_174874" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2510009N07Rik; Apg4b; Atg4bl; Autl1; AW048066 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206129 representing NM_174874
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCAGCCACTTTGACATATGATACTCTCCGGTTTGCTGAATTTGAAGATTTCCCTGAGACCTCAG AGCCTGTTTGGATTCTGGGCAGAAAATACAGCATTTTCACAGAGAAGGACGAAATCTTGTCTGATGTTGC ATCCAGACTTTGGTTTACATACAGGAGAAACTTTCCAGCTATTGGGGGAACTGGCCCTACTTCAGACACA GGCTGGGGTTGCATGCTTCGGTGTGGACAGATGATCTTTGCCCAGGCCCTGGTATGCCGGCACTTAGGTC GAGATTGGAGGTGGACTCAGCGGAAGAGGCAGCCTGACAGCTACTTTAATGTCCTCAATGCTTTCCTCGA CAGGAAGGACAGCTACTATTCCATCCATCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCTATAGGC CAGTGGTACGGGCCGAACACTGTTGCCCAGGTCCTCAAGAAACTTGCTGTATTCGACACATGGAGTTCCT TGGCTGTTCACATAGCAATGGACAACACTGTGGTGATGGAGGAAATCAGAAGGTTATGCAGGGCCAACCT TCCCTGTGCTGGGGCGGCTGCACTTCCTACTGATTCAGAGAGGCACTGTAATGGGTTCCCTGCTGGAGCT GAAGTCACCAACAGGCCATCGGCTTGGAGACCACTAGTGCTTCTCATCCCTCTCCGCCTGGGACTGACAG ACATCAATGAGGCCTATGTGGAGACGCTGAAGCACTGTTTCATGATGCCCCAGTCCCTGGGTGTTATTGG AGGGAAGCCCAACAGTGCCCACTACTTTATTGGCTATGTTGGGGAGGAGCTCATCTATCTGGACCCCCAC ACTACACAGCCTGCAGTGGAGCTGACTGACAGCTGCTTCATCCCCGACGAGAGCTTCCACTGCCAGCACC CTCCCTCTAGGATGGGCATTGGAGAGCTTGACCCGTCCATTGCTGTGGGGTTTTTCTGTAAGAAGGAGGA AGACTTTAACGACTGGTGTCAGCAAGTCAAAAAGCTATCCCAGCTTGGAGGTGCCCTGCCCATGTTTGAG CTGGTGGAACAGCAGCCTTCCCATCTGGCCTGCCAGGATGTCCTGAACCTGTCCCTAGACTCTTCTGATG TAGAGAGACTGGAGAGGTTTTTTGACTCTGAGGATGAAGACTTTGAAATCTTATCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206129 representing NM_174874
Red=Cloning site Green=Tags(s) MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRRNFPAIGGTGPTSDT GWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFNVLNAFLDRKDSYYSIHQIAQMGVGEGKSIG QWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRANLPCAGAAALPTDSERHCNGFPAGA EVTNRPSAWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPH TTQPAVELTDSCFIPDESFHCQHPPSRMGIGELDPSIAVGFFCKKEEDFNDWCQQVKKLSQLGGALPMFE LVEQQPSHLACQDVLNLSLDSSDVERLERFFDSEDEDFEILSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174874 |
| ORF Size | 1179 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174874.1, NM_174874.2, NM_174874.3, NP_777363.1 |
| RefSeq Size | 2965 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 66615 |
| UniProt ID | Q8BGE6 |
| MW | 44.9 kDa |
| Gene Summary | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210405 | Atg4b (untagged) - Mouse autophagy-related 4B (yeast) (Atg4b), (10ug) |
CNY 5,488.00 |
|
| MG206129 | Atg4b (tGFP-tagged) - Mouse autophagy-related 4B (yeast) (Atg4b) |
CNY 7,088.00 |
|
| MR206129L3 | Lenti ORF clone of Atg4b (Myc-DDK-tagged) - Mouse autophagy-related 4B (yeast) (Atg4b) |
CNY 5,890.00 |
|
| MR206129L4 | Lenti ORF clone of Atg4b (mGFP-tagged) - Mouse autophagy-related 4B (yeast) (Atg4b) |
CNY 5,890.00 |
