Prmt6 (NM_178891) Mouse Tagged ORF Clone
CAT#: MR205897
- TrueORF®
Prmt6 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 6 (Prmt6)
ORF Plasmid: tGFP
"NM_178891" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW124876; BB233495; Hrmt1l6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205897 representing NM_178891
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGAGCAAGAAAAGAAAGCTTGAGTCGGGGGACAGCGGAGGCGCCGGCGCCGGAGGGGAGGGAG CCGAGGAGGAAAATGGCGGGGAGCAGGAGGCGGCCCCGCCACGACCCCGGAGGACCAAGAGCGAGCGGGA CCAGCTGTACTACGAGTGCTACTCCGACGTCTCGGTCCACGAGGAGATGATCGCCGACCAGGTCCGCACC GAAGCCTACCGCTTAGGCATCCTGAAGAACTGGGCCGCGCTGCGAGGCAAGACGGTGCTGGACGTGGGCG CGGGCACCGGCATTCTCAGCATCTTCTGTGCCCAGGCCGGGGCACGGCGCGTGTACGCGGTGGAGGCCAG CGCCATCTGGCAACAGGCCCGGGAGGTGGTGCGGCTCAACGGGTTGGAGGACCGCGTGCACGTCCTGCCG GGCCCGGTGGAGACCGTGGAGCTGCCGGAGCGAGTGGACGCCATCGTCAGCGAGTGGATGGGCTACGGAC TTCTGCACGAGTCCATGCTGAGCTCCGTGCTCCACGCGCGGACCAAATGGCTGAAGGAGGGCGGTCTCCT CCTGCCAGCTTCCGCGGAGCTCTTCGTGGCCCCGATTAGCGACCAGATGCTCGAGTGGCGTCTGGGTTTC TGGAGCCAGGTGAAGCAGCACTATGGCGTGGATATGAGCTGCATGGAGAGCTTCGCCACGCGCTGCCTCA TGGGCCATTCGGAGATCGTGGTGCAGGATCTGTCCGGAGAGGACGTGCTGGCCCGGCCGCAGCGCTTTGC CCAGCTCGAGCTGGCCCGAGCCGGCCTGGAGCAGGAGCTGGAGGCTGGTGTGGGCGGGCGCTTCCGCTGC AGCTGCTATGGTTCCGCGCCTCTACATGGTTTCGCCGTCTGGTTTCAAGTGACCTTTCCCGGAGGGGACT CGGAGAAACCTCTGGTGCTGTCCACCTCGCCTTTTCACCCGGCCACCCACTGGAAGCAGGCGCTCCTCTA CTTGAACGAGCCGGTGCCGGTGGAACAAGATACGGACATTTCCGGAGAGATCACCCTGCTGCCCTCCCCG GACAACCCCCGGCGTCTGCGCATACTTCTGCGCTACAAAGTGGGAGACCATGAGGAAAAGACCAAAGACT TTGCCATGGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205897 representing NM_178891
Red=Cloning site Green=Tags(s) MSLSKKRKLESGDSGGAGAGGEGAEEENGGEQEAAPPRPRRTKSERDQLYYECYSDVSVHEEMIADQVRT EAYRLGILKNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRLNGLEDRVHVLP GPVETVELPERVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFVAPISDQMLEWRLGF WSQVKQHYGVDMSCMESFATRCLMGHSEIVVQDLSGEDVLARPQRFAQLELARAGLEQELEAGVGGRFRC SCYGSAPLHGFAVWFQVTFPGGDSEKPLVLSTSPFHPATHWKQALLYLNEPVPVEQDTDISGEITLLPSP DNPRRLRILLRYKVGDHEEKTKDFAMED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178891 |
ORF Size | 1134 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178891.5, NP_849222.3 |
RefSeq Size | 2475 bp |
RefSeq ORF | 1137 bp |
Locus ID | 99890 |
UniProt ID | Q6NZB1 |
MW | 42.3 kDa |
Gene Summary | Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed:22904064, PubMed:26070566). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (By similarity). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (By similarity). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (By similarity). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed:22904064). Repression of TP53 blocks cellular senescence (PubMed:22904064). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (PubMed:24570487). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (PubMed:26070566).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212048 | Prmt6 (untagged) - Mouse protein arginine N-methyltransferase 6 (Prmt6), (10ug) |
CNY 3,990.00 |
|
MG205897 | Prmt6 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 6 (Prmt6) |
CNY 3,140.00 |
|
MR205897L3 | Lenti ORF clone of Prmt6 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 6 (Prmt6) |
CNY 4,750.00 |
|
MR205897L4 | Lenti ORF clone of Prmt6 (mGFP-tagged) - Mouse protein arginine N-methyltransferase 6 (Prmt6) |
CNY 4,750.00 |