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Akap10 (BC054105) Mouse Tagged Lenti ORF Clone
CAT＃: MR205740L4
- LentiORF®
Lenti ORF clone of Akap10 (mGFP-tagged) - Mouse A kinase (PRKA) anchor protein 10 (cDNA clone MGC:60489 IMAGE:30022728)
"BC054105" in other vectors (4)
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CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 1500031L16Rik; B130049N18Rik; D-AKAP-2; D-AKAP2; PRKA10 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR205740).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC054105 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054105.1 |
| RefSeq Size | 2836 bp |
| RefSeq ORF | 1118 bp |
| Locus ID | 56697 |
| Gene Summary | This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. It has been reported that this protein is important for maintaining heart rate and myocardial contractility through its targeting of protein kinase A. In mouse, defects of this gene lead to cardiac arrhythmias and premature death. In humans, polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218334 | Akap10 (untagged) - Mouse A kinase (PRKA) anchor protein 10 (cDNA clone MGC:60489 IMAGE:30022728), (10ug) |
CNY 6,940.00 |
|
| MG205740 | Akap10 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 10 (cDNA clone MGC:60489 IMAGE:30022728) |
CNY 4,090.00 |
|
| MR205740 | Akap10 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 10 (cDNA clone MGC:60489 IMAGE:30022728) |
CNY 3,710.00 |
|
| MR205740L3 | Lenti ORF clone of Akap10 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 10 (cDNA clone MGC:60489 IMAGE:30022728) |
CNY 5,610.00 |
