B3gnt3 (NM_028189) Mouse Tagged ORF Clone
CAT#: MR205739
- TrueORF®
B3gnt3 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 (B3gnt3)
ORF Plasmid: tGFP
"NM_028189" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2210008L19Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205739 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTGCCAAGGCAGAGCCCCTACGAGATCCTCCTCCTGGTCTTGGTCGCCTTGCTGGTGCTGCTGC TGCTCCTGACCAGCAAGTCACCGCCCAGCTGCTCCGCCCCTGAGAGGTCCAAGGAGCCTGAAGACAACCC CGGGTGGGCCACGGGCCACCCACGCACACACCCCGCCCGGTGCCGAGCTAATCTATCCGTGTCCTCGCAC CCCGACTTCGCGGGGCTGCCCTTGCACGTGCGCGACTTCTTGTTCTACCGCCACTGCCGCGACTTCCCAG TGCTCCGAGAGCCGCGGGTTACCAAGTGCGCGGAGCCCGTGTTCCTGCTGCTCGCCATCAAGTCCTCGCC TGCAAACTATGGGCGCCGCCAGATGCTGCGCACGACGTGGGCGCGCGAGAGACGGGTGCGTGGGGCGCCA CTGCGCCGCCTCTTCCTTGTGGGCTCAGACCGCGACCCACAACAAGCACGCAAATACAACCGACTGCTGG AGCTGGAAGCGCAGAAATACGGCGACATTCTCCAGTGGGATTTCCATGACTCCTTCTTTAACCTGACGCT TAAGCAGGTCCTTTTCCTGGAGTGGCAGCTAACCTACTGTACCAACGCCAGCTTCGTGCTCAATGGGGAC GACGATGTGTTCGCACACACGGACAACATGGTCACCTACCTGCAGGACCACGACCCGGACCAACACCTCT TCGTGGGGCACCTGATCCAGAACGTGGGTCCCATCCGGGTGCCCTGGAGCAAGTACTTCATCCCCACTCT GGTGATGGCGGAGGACAGATACCCGCCCTACTGTGGTGGCGGCGGCTTCCTGCTGTCGCGTTTTACCGTG GCCGCCCTACGTCGCGCCGCGCGCGTCCTCCCCATGTTCCCAATCGACGACGTGTTCCTGGGCATGTGTC TGCAGCAGCAGGGGCTGGCTCCCGGGACGCACAGCGGAGTGCGCACTGCGGGGGTTTTCCCCCCTAGCCC ACGTGTGTCATCCTTCGACCCCTGCTTCTACCGCGACCTGCTCCTCGTGCACCGCTTCCTGCCCTTCGAG ATGCTGCTGATGTGGGATGCGCTGAACCAGCCCCAGCTCCTCTGCGGCAGGCAGAGCCCCGCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205739 protein sequence
Red=Cloning site Green=Tags(s) MRLPRQSPYEILLLVLVALLVLLLLLTSKSPPSCSAPERSKEPEDNPGWATGHPRTHPARCRANLSVSSH PDFAGLPLHVRDFLFYRHCRDFPVLREPRVTKCAEPVFLLLAIKSSPANYGRRQMLRTTWARERRVRGAP LRRLFLVGSDRDPQQARKYNRLLELEAQKYGDILQWDFHDSFFNLTLKQVLFLEWQLTYCTNASFVLNGD DDVFAHTDNMVTYLQDHDPDQHLFVGHLIQNVGPIRVPWSKYFIPTLVMAEDRYPPYCGGGGFLLSRFTV AALRRAARVLPMFPIDDVFLGMCLQQQGLAPGTHSGVRTAGVFPPSPRVSSFDPCFYRDLLLVHRFLPFE MLLMWDALNQPQLLCGRQSPAY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028189 |
ORF Size | 1119 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028189.2, NP_082465.2 |
RefSeq Size | 2103 bp |
RefSeq ORF | 1119 bp |
Locus ID | 72297 |
UniProt ID | Q5JCS9 |
MW | 42.6 kDa |
Gene Summary | Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202991 | B3gnt3 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 (B3gnt3), (10ug) |
CNY 3,656.00 |
|
MG205739 | B3gnt3 (tGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 (B3gnt3) |
CNY 3,710.00 |
|
MR205739L3 | Lenti ORF clone of B3gnt3 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 (B3gnt3) |
CNY 5,230.00 |
|
MR205739L4 | Lenti ORF clone of B3gnt3 (mGFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 (B3gnt3) |
CNY 5,230.00 |