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Cdk9 (NM_130860) Mouse Tagged ORF Clone
CAT＃: MR205733
- TrueORF®
Cdk9 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 9 (CDC2-related kinase) (Cdk9)
ORF Plasmid: tGFP
"NM_130860" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PITALRE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205733 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGCAGTACGACTCGGTGGAATGCCCGTTCTGCGATGAGGTCACCAAGTACGAGAAACTTGCCA AGATCGGCCAAGGCACATTCGGGGAAGTCTTTAAAGCCAAGCACCGTCAGACGGGCCAGAAGGTGGCTCT GAAGAAAGTGTTGATGGAGAATGAGAAGGAGGGGTTCCCCATCACAGCCTTGAGGGAAATCAAGATTCTA CAGCTCCTAAAACATGAGAATGTGGTGAACCTAATTGAGATTTGTCGGACCAAAGCCTCACCGTATAACC GCTGCAAGGGCAGCATCTATCTGGTGTTTGACTTCTGTGAGCATGACCTTGCTGGGCTGCTGAGCAACGT CTTAGTCAAGTTCACGTTGTCTGAGATCAAGAGAGTGATGCAGATGCTGCTGAACGGCCTCTACTACATC CACAGGAACAAGATCCTGCACAGGGACATGAAGGCTGCGAATGTGCTCATTACCCGGGATGGGGTCCTGA AGCTGGCAGATTTTGGGCTGGCCCGTGCCTTCAGCCTAGCTAAGAATAGCCAGCCCAACCGATACACCAA CCGTGTGGTGACATTGTGGTACCGGCCTCCGGAGCTGCTGCTCGGAGAGCGGGACTACGGCCCGCCCATT GACCTTTGGGGTGCTGGGTGCATCATGGCAGAGATGTGGACTCGTAGCCCTATCATGCAGGGTAACACAG AGCAGCACCAGCTCGCCCTCATCAGCCAGCTCTGTGGCTCCATCACCCCAGAGGTGTGGCCAAACGTAGA CAAGTATGAGCTGTTTGAAAAGCTGGAACTTGTGAAGGGCCAGAAGCGGAAGGTGAAGGACCGTCTGAAG GCCTACGTGCGCGACCCCTATGCTCTGGACCTCATTGACAAGCTGCTGGTGCTGGACCCCGCGCAGCGGA TTGACAGTGACGATGCCCTCAACCACGACTTCTTCTGGTCGGACCCCATGCCCTCGGACCTCAAGGGCAT GCTGTCTACTCACTTGACGTCTATGTTTGAGTACCTGGCACCGCCACGGCGGAAGGGCAGCCAGATCACC CAGCAGTCCACCAACCAGAGCAGAAATCCCGCCACCACCAACCAGACGGAATTTGAACGTGTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205733 protein sequence
Red=Cloning site Green=Tags(s) MAKQYDSVECPFCDEVTKYEKLAKIGQGTFGEVFKAKHRQTGQKVALKKVLMENEKEGFPITALREIKIL QLLKHENVVNLIEICRTKASPYNRCKGSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYI HRNKILHRDMKAANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLLGERDYGPPI DLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDKYELFEKLELVKGQKRKVKDRLK AYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQIT QQSTNQSRNPATTNQTEFERVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130860 |
| ORF Size | 1119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130860.3 |
| RefSeq Size | 3389 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 107951 |
| UniProt ID | Q99J95 |
| MW | 42.8 kDa |
| Gene Summary | Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200590 | Cdk9 (untagged) - Mouse cyclin-dependent kinase 9 (CDC2-related kinase) (Cdk9), (10ug) |
CNY 3,656.00 |
|
| MG205733 | Cdk9 (tGFP-tagged) - Mouse cyclin-dependent kinase 9 (CDC2-related kinase) (Cdk9) |
CNY 3,710.00 |
|
| MR205733L3 | Lenti ORF clone of Cdk9 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 9 (CDC2-related kinase) (Cdk9) |
CNY 5,230.00 |
|
| MR205733L4 | Lenti ORF clone of Cdk9 (mGFP-tagged) - Mouse cyclin-dependent kinase 9 (CDC2-related kinase) (Cdk9) |
CNY 5,230.00 |
