United States
Germany
Japan
United Kingdom
China
Ccny (BC023321) Mouse Tagged ORF Clone
CAT＃: MR205108
- TrueORF®
Ccny (Myc-DDK-tagged) - Mouse cyclin Y (cDNA clone MGC:28252 IMAGE:3995040)
ORF Plasmid: tGFP
"BC023321" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700025H17Rik; 3110050L10Rik; 4631402G10Rik; 5730405I09Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205108 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACACAACTTCGTGCTGCGTGTCGTCCAGCCCCAAGCTCCGGAGGAATGCGCACTCGCGGCTGG AGTCCTACCGCCCGGACACGGACCTCAGCCGTGAGGACACTGGCTGCAATCTGCAGCACATCAGCGACCG GGAAAACATCGACGATTTAAACATGGAATTCAATCCTTCAGACCATCCTCGGGCCAGCACAATATTCCTC AGCAAATCTCAGACAGACGTGAGAGAAAAACGCAAGAGTCTCTTCATTAACCATCATCCTCCAGGGCAGA CATCAAGGAAATACAGCTCTTGCTCCACCATCTTCCTAGATGACAGCACAGTCAGCCAGCCAAACCTCAA GTACACCATCAAATGTGTAGCTCTTGCGATATATTACCACATCAAAAACAGGGACCCAGATGGAAGGATG CTCTTAGATATTTTTGATGAAAACCTCCACCCTCTTTCGAAATCCGAAGTGCCACCTGATTATGACAAAC ACAACCCAGAACAGAAGCAAATTTACCGGTTCGTTCGGACACTCTTCAGTGCTGCTCAGCTGACAGCTGA ATGTGCCATTGTCACCCTGGTATACCTTGAAAGGCTCTTGACATACGCAGAGATTGATATCTGTCCAGCC AATTGGAAGCGCATTGTTCTAGGGGCAATCCTGCTGGCCTCTAAGGTGTGGGATGACCAAGCTGTGTGGA ATGTGGATTACTGCCAGATCCTGAAAGACATCACAGTGGAGGACATGAATGAACTGGAGAGGCAGTTTCT TGAATTGCTACAGTTTAACATCAATGTTCCTTCCAGTGTGTATGCCAAGTATTATTTTGATCTTCGTTCT CTGGCAGAAGCAAACAACCTGAGCTTTCCTCTGGAGCCCCTAAGCAGAGAGCGTGCCCACAAGCTTGAGG CCATCTCTCGTCTCTGTGAGGACAAGTACAAGGACCTGAGGAAACCCATGAGGAAGCGTTCAGCAAGTGC TGACAACCTGATTCTGCCAAGGTGGTCTCCAGCCATCATCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205108 protein sequence
Red=Cloning site Green=Tags(s) MGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFL SKSQTDVREKRKSLFINHHPPGQTSRKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRM LLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPA NWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRS LAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRKPMRKRSASADNLILPRWSPAIIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023321 |
| ORF Size | 1023 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023321, AAH23321 |
| RefSeq Size | 2104 bp |
| RefSeq ORF | 1025 bp |
| Locus ID | 67974 |
| MW | 39.4 kDa |
| Gene Summary | Positive regulatory subunit of the cyclin-dependent kinase CDK14/PFTK1. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway (By similarity). Recruits CDK16 to the plasma membrane (By similarity). Positive regulatory subunit of the cyclin-dependent kinase CDK16.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217846 | Ccny (untagged) - Mouse cyclin Y (cDNA clone MGC:28252 IMAGE:3995040), (10ug) |
CNY 6,940.00 |
|
| MG205108 | Ccny (tGFP-tagged) - Mouse cyclin Y (cDNA clone MGC:28252 IMAGE:3995040) |
CNY 2,090.00 |
|
| MR205108L3 | Lenti ORF clone of Ccny (Myc-DDK-tagged) - Mouse cyclin Y (cDNA clone MGC:28252 IMAGE:3995040) |
CNY 3,800.00 |
|
| MR205108L4 | Lenti ORF clone of Ccny (mGFP-tagged) - Mouse cyclin Y (cDNA clone MGC:28252 IMAGE:3995040) |
CNY 3,800.00 |
