United States
Germany
Japan
United Kingdom
China
Ankrd32 (BC009101) Mouse Tagged ORF Clone
CAT＃: MR205104
- TrueORF®
Ankrd32 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 32 (cDNA clone MGC:12146 IMAGE:3710846)
ORF Plasmid: tGFP
"BC009101" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,420.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700017A04Rik; Ankrd32; AW545819; Brctd1; Brctx; C730024G01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205104 representing BC009101
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATACATGGGGCTGGAAAGATGCAGAGTCCAAAAAAACTCTGCCAGCGGCCTTGCCTAGAGTCTC AAAGAGCATTACTAATGCTGAATGGTGCAAAACGGAAACAAGCAGAAGGTCGGCCAGAGCTCCTAGAGTT AAACCGTGCTAAATGTTCCTCATCATTGAAAAAATTGAAAAAGAAGTCAGAGGAATTGTCATGTTCCAAG GAGAATTGCCCATCTTTGGTTACAAAGATGAATTTTCATAAAACTAATCTGAAAGGGGAAACAGCTCTGC ATAGAGTTTGCATAAAAAACCAAGTGGAGAAATTGATTATCCTTCTGTCTTTGCCAGGAATAGACATCAA TGTTAAAGACAATGCTGGCTGGACGCCTTTGCATGAAGCCTGCAACTATGGCAACACGGAGTGTGTCCAG GAAATTCTGCAGCGTTGTCCAGAGGTGGATCTGCTCACTCAAGTGGACGGGGTGACGCCTTTGCATGATG CGCTGTCAAACGGACATGTGGAGATTGGCAAGCTGCTTCTACAGCGTGGGGGCCCAGAACTTTTACAGCA GAGGAACTCTAAGGGGGAGTTGCCCTTGGATTATGTGCTGTCCCCTAAGGACAAGGAAGAACTATTTGCC ATTACAAATATAGACGATACAGTGGACAACTTTCATGCTAAGACACAGAAACATTTTTACCACCAACAGC TTGAATTTGGTTCGTTTTTACTTAGTAGGATGTTGATTAATTTTTGTTCAATATTTGATTTGTCCTCAGA ATTTATCCTAGCTTTCAAAGGGTTAGGTCATCTAAATGAGTTGCTTATGGCATGCAATAGTGATACAGAA GCCAGCAATGCACATACTGACTGGTTATTGGATGTTTATGCTAGGAATATAAAGACATTAAAGAAGCTCC CAAGTGTTCTTAAAGAGCTGCCTGAGAATCTAAATGTATGTCCTGGAGTGCATACTGAGGCCTTACTGGT GACACTGAAAATGATGTGTCAGTCAATCACAGAGCTGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205104 representing BC009101
Red=Cloning site Green=Tags(s) MDIHGAGKMQSPKKLCQRPCLESQRALLMLNGAKRKQAEGRPELLELNRAKCSSSLKKLKKKSEELSCSK ENCPSLVTKMNFHKTNLKGETALHRVCIKNQVEKLIILLSLPGIDINVKDNAGWTPLHEACNYGNTECVQ EILQRCPEVDLLTQVDGVTPLHDALSNGHVEIGKLLLQRGGPELLQQRNSKGELPLDYVLSPKDKEELFA ITNIDDTVDNFHAKTQKHFYHQQLEFGSFLLSRMLINFCSIFDLSSEFILAFKGLGHLNELLMACNSDTE ASNAHTDWLLDVYARNIKTLKKLPSVLKELPENLNVCPGVHTEALLVTLKMMCQSITELS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC009101 |
| ORF Size | 1020 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC009101.1 |
| RefSeq Size | 1601 bp |
| RefSeq ORF | 1022 bp |
| Locus ID | 105377 |
| MW | 38.1 kDa |
| Gene Summary | Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205104 | Ankrd32 (tGFP-tagged) - Mouse ankyrin repeat domain 32 (cDNA clone MGC:12146 IMAGE:3710846) |
CNY 3,800.00 |
|
| MR205104L3 | Lenti ORF clone of Ankrd32 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 32 (cDNA clone MGC:12146 IMAGE:3710846) |
CNY 5,320.00 |
|
| MR205104L4 | Lenti ORF clone of Ankrd32 (mGFP-tagged) - Mouse ankyrin repeat domain 32 (cDNA clone MGC:12146 IMAGE:3710846) |
CNY 5,320.00 |
其它Ankrd32产品
