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Etfa (NM_145615) Mouse Tagged ORF Clone
CAT＃: MR204913
- TrueORF®
Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_145615" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010200I21Rik; D9Ertd394e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204913 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGAGCAGCGGCGCCTGGGCAGCTCCGGCGGGCGGCCTCATTGCTCCGTTTTCAGAGTACCTTGG TAATAGCTGAGCATGCAAATGATTCCCTAGCACCCATTACTCTAAATACTATCACTGCAGCTGGACGTCT TGGAGGTGAAGTGTCCTGCTTAGTAGCTGGAACCAAGTGTGACAAGGTGGTACAGGATCTATGTAAAGTA GCTGGCGTAGCAAAGGTTCTGGTGGCTCAGCATGATGCATACAAAGGCCTCCTTCCAGAGGAACTCACAC CGTTGATTTTGGAAACTCAGAAGCAGTTCAGTTACACACACATCTGTGCTGGAGCGTCTGCTTTTGGAAA GAACCTTCTGCCCAGAGTAGCTGCCAAACTTAATGTTGCTCCAGTTTCTGACATCATTGAGATCAAGTCA CCTGACACATTTGTGAGAACTATCTATGCAGGAAATGCGTTGTGTACAGTAAAGTGTGATGAGAAAGTAA AGGTGTTTTCTGTTCGAGGAACATCTTTTGAGGCTGCAGCAACAAGTGGAGGTAGTGCTAGTTCAGAAAA GGCACCAAGTTCTTCATCAGTGGGAATATCAGAGTGGCTTGACCAGAAATTGACAAAAAGTGACCGACCA GAACTAACTGGTGCCAAAGTGGTGGTATCTGGTGGTCGGGGTTTGAAGAGTGGAGAGAACTTTAAACTGC TGTATGACCTGGCAGATCAACTACATGCTGCAGTTGGTGCTTCCCGAGCTGCTGTTGATGCTGGCTTTGT TCCCAATGACATGCAAGTTGGTCAGACAGGAAAAATAGTAGCTCCAGAACTTTATATTGCTGTTGGAATA TCTGGAGCCATTCAACATTTAACTGGGATGAAGGACAGCAAGACAATCGTGGCTATTAACAAAGATCCAG AAGCTCCAATTTTCCAGGTGGCAGATTATGGAATAGTTGCAGATTTATTTAAGGTGGTTCCTGAAATGAC AGAAATACTGAAGAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204913 protein sequence
Red=Cloning site Green=Tags(s) MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAAGRLGGEVSCLVAGTKCDKVVQDLCKV AGVAKVLVAQHDAYKGLLPEELTPLILETQKQFSYTHICAGASAFGKNLLPRVAAKLNVAPVSDIIEIKS PDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFEAAATSGGSASSEKAPSSSSVGISEWLDQKLTKSDRP ELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGI SGAIQHLTGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145615 |
| ORF Size | 1002 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145615.1 |
| RefSeq Size | 1464 bp |
| RefSeq ORF | 1002 bp |
| Locus ID | 110842 |
| UniProt ID | Q99LC5 |
| MW | 35 kDa |
| Gene Summary | Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212235 | Etfa (untagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
| MG204913 | Etfa (tGFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein |
CNY 3,140.00 |
|
| MR204913L3 | Lenti ORF clone of Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| MR204913L4 | Lenti ORF clone of Etfa (mGFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
