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Akr1c21 (NM_029901) Mouse Tagged ORF Clone
CAT＃: MR204697
- TrueORF®
Akr1c21 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member C21 (Akr1c21)
ORF Plasmid: tGFP
"NM_029901" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9430025F20Rik; AI315367 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204697 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCAAATGTCATTGTGTCATATTGAATGATGGTAACTTCATTCCAGTGCTGGGTTTTGGTACTG CTCTTCCTGTAGAGTGTCCCAAGAGTAAAGCTAAGGAGCTCACCAAAATAGCTATAGATGCTGGTTTCCA TCACTTTGATTCTGCTTCTGTCTATAATACCGAAGATCGTGTAGGAGAGGCCATCAGAAGCAAGATTGCT GATGGCACTGTAAGGAGAGAAGATATATTTTACACCTCAAAGGTTTGGTGTACTAGCCTTCGCCCAGAAC TTGTGAGAGCTTCCTTGGTACGGTCACTGCAAAAACTTCAGTTCGATTATGTGGACCTGTATCTCATTCA TTACCCAATGGCCCTGAAACCAGGAGAAGAAAATTTTCCAGTAGATGAACATGGAAAATTAATATTTGAC AGAGTGGACCTCTGTGCCACCTGGGAGGCCATGGAGAAGTGTAAGGATGCAGGACTAACCAAGTCCATTG GGGTGTCTAACTTTAACTCTAGACAGTTGGAGATGATTCTGAATAAGCCTGGGCTCAAGTACAAGCCGGT ATGCAACCAGGTAGAATGCCATCCTTATCTCAACCAAATGAAACTTCTGGATTTCTGCAAATCAAAAGAT ATTGTATTGGTTGCCTATGGTGTTCTAGGAACACAACGATATGGAGGATGGGTAGACCAGAATTCCCCTG TTCTCTTGGATGAACCAGTTCTTGGTTCCATGGCAAAAAAATATAATCGAACTCCAGCCTTGATTGCCCT TCGCTACCAGTTACAGCGTGGGATTGTGGTCCTCAACACCAGTCTCAAAGAGGAGCGGATCAAAGAGAAC ATGCAGGTTTTTGAATTCCAGCTGAGTTCAGAGGATATGAAAGTTCTTGATGGCCTGAACAGAAATATGC GATACATACCTGCTGCCATTTTCAAGGGCCACCCTAATTGGCCATTTTTGGATGAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204697 protein sequence
Red=Cloning site Green=Tags(s) MNSKCHCVILNDGNFIPVLGFGTALPVECPKSKAKELTKIAIDAGFHHFDSASVYNTEDRVGEAIRSKIA DGTVRREDIFYTSKVWCTSLRPELVRASLVRSLQKLQFDYVDLYLIHYPMALKPGEENFPVDEHGKLIFD RVDLCATWEAMEKCKDAGLTKSIGVSNFNSRQLEMILNKPGLKYKPVCNQVECHPYLNQMKLLDFCKSKD IVLVAYGVLGTQRYGGWVDQNSPVLLDEPVLGSMAKKYNRTPALIALRYQLQRGIVVLNTSLKEERIKEN MQVFEFQLSSEDMKVLDGLNRNMRYIPAAIFKGHPNWPFLDEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029901 |
| ORF Size | 972 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029901.1, NM_029901.2, NP_084177.2 |
| RefSeq Size | 1222 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 77337 |
| UniProt ID | Q91WR5 |
| MW | 36.8 kDa |
| Gene Summary | NADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into androsterone. Has lower 3-alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha-hydroxysteroids, 17-ketosteroids, 3-alpha hydroxysteroids and 3-ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17-alpha-hydroxysteroids. Likewise, reduction of 3-ketosteroids yields only 3-alpha-hydroxysteroids.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203832 | Akr1c21 (untagged) - Mouse aldo-keto reductase family 1, member C21 (Akr1c21), (10ug) |
CNY 2,400.00 |
|
| MG204697 | Akr1c21 (tGFP-tagged) - Mouse aldo-keto reductase family 1, member C21 (Akr1c21) |
CNY 2,850.00 |
|
| MR204697L3 | Lenti ORF clone of Akr1c21 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member C21 (Akr1c21) |
CNY 4,750.00 |
|
| MR204697L4 | Lenti ORF clone of Akr1c21 (mGFP-tagged) - Mouse aldo-keto reductase family 1, member C21 (Akr1c21) |
CNY 4,750.00 |
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