Dusp7 (BC010207) Mouse Tagged ORF Clone

CAT#: MR204628

  • TrueORF®

Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527)

ORF Plasmid: DDK tGFP



  "BC010207" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 6,365.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MKPX, MKP-X, PYST2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204628 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCATCATTCCCAACCATGCCGACAAGGAGC
GCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACGGCGGAGTGGCAGCCCGA
GCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGCTGCGAGACGACGGCTGCCAGGCCTAC
TACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCTGAGCATTGTGAGACTAACGTGGACAGCT
CGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTG
CTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTGAGTCGGATGGCAGCCCTGTACCATCC
AGCCAGCCGGCCTTTCCGGTCCAGATCCTGCCCTACCTCTACCTCGGCTGTGCCAAGGACTCTACCAACC
TGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTCACACCCAACCTGCCCAACGCCTTTGA
GCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACCACTGGAGCCAGAACCTCTCTCAGTTC
TTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAAGTGTGGTGTCTTGGTGCACTGCCTAG
CAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATGCAGAAGATGAACCTGTCCCTCAATGA
TGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTG
GACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCACGCACCCAGTGAGCAGCTCTACTTCT
CCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAGTCCACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204628 protein sequence
Red=Cloning site Green=Tags(s)

MLRRLRKGNLPIRSIIPNHADKERFATRCKAATVLLYDEATAEWQPEPGAPASVLGLLLQKLRDDGCQAY
YLQGGFNKFQTEYSEHCETNVDSSSSPSGSPPTSVLGLGGLRISSDCSDGESDRELPSSATESDGSPVPS
SQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNVTPNLPNAFEHGGEFTYKQIPISDHWSQNLSQF
FPEAISFIDEARSKKCGVLVHCLAGISRSVTVTVAYLMQKMNLSLNDAYDFVKRKKSNISPNFNFMGQLL
DFERTLGLSSPCDNHAPSEQLYFSTPTNHNLFPINTLEST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC010207
ORF Size 960 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010207, AAH10207
RefSeq Size 2584 bp
RefSeq ORF 962 bp
Locus ID 235584
MW 35.3 kDa
Gene Summary Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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