Krt13 (BC060286) Mouse Tagged ORF Clone

CAT#: MR204414

  • TrueORF®

Krt13 (Myc-DDK-tagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772)

ORF Plasmid: DDK tGFP



  "BC060286" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CK13, K13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204414 representing BC060286
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGCCGCTTCCAGAGTTCCTCCATGAGCTATGGAGGTGGTTTCGGAGCTGGTTCTTGCCAGCTTG
GAGGAGGCCGTAATATCTCCTCCTGCTCATCTCGGTTTGTCACTGGAGGGTCAGCTGGAGGCTATGGAGG
GGGCATGAGCTGTGGCTTTGGTGGAGGAGCTGGTGGTGGCTTTGGAGGTGGCTTTGGAGGTGGCTTTGGA
GGTAGCTATGGCGGAGGTTTTGGCGGTGGCTTTGGTGACTTCGGAGGTGTCGATGGTGGCCTCCTCTCTG
GCAACGAGAAGATCACCATGCAGAACCTCAATGACCGCCTGGCCAGCTACCTGGATAAGGTGCGCGCCTT
GGAGGCAGCCAATGCCGACCTGGAGGTGAAGATTCGTGACTGGCATCTGAAACAGAGCCCAGCTAGTCCA
GAGCGGGACTACAGTGCTTATTATAAGACCATCGAGGAGCTCCGGATCAAGATTCTGGAAGCCACCACGG
ACAACAACCGGATCATCCTGGAGATTGACAATGCCAGGCTGGCTGCAGATGACTTCAGGCTCAAGTACGA
AAATGAGCTGACCTTGCGCCAGAGCGTGGAGGCCGACATCAACGGCCTGCGCAGGGTGCTGGATGAGCTG
ACTCTGGCTAAGACTGACCTGGAAATGCAGATTGAGAGCCTGAATGAAGAACTGGCTTACCTGAAGAAGA
ACCACGAAGAGGAGATGAAGGAATTCAGCAACCAGGTAGTAGGCCAGGTCAATGTGGAGATGGACGCCAC
CCCTGGCATTGATCTGACCCGTGTGCTGGCTGAGATGAGGGAGCAGTATGAAGCCCTGGCAGAGAAGAAT
CGGAGGGATGCTGAGGAATGGTTCCAGACCAAGAGTGCAGAGCTGAACAAGGAAGTATCCTCCAATTCCG
GTCGCCCAGATTTCCGAAAGTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204414 representing BC060286
Red=Cloning site Green=Tags(s)

MSCRFQSSSMSYGGGFGAGSCQLGGGRNISSCSSRFVTGGSAGGYGGGMSCGFGGGAGGGFGGGFGGGFG
GSYGGGFGGGFGDFGGVDGGLLSGNEKITMQNLNDRLASYLDKVRALEAANADLEVKIRDWHLKQSPASP
ERDYSAYYKTIEELRIKILEATTDNNRIILEIDNARLAADDFRLKYENELTLRQSVEADINGLRRVLDEL
TLAKTDLEMQIESLNEELAYLKKNHEEEMKEFSNQVVGQVNVEMDATPGIDLTRVLAEMREQYEALAEKN
RRDAEEWFQTKSAELNKEVSSNSGRPDFRKY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060286
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060286.1
RefSeq Size 1232 bp
RefSeq ORF 935 bp
Locus ID 16663
MW 45.1 kDa
Gene Summary The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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