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Tyms (BC020139) Mouse Tagged ORF Clone
CAT＃: MR204343
- TrueORF®
Tyms (Myc-DDK-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204)
ORF Plasmid: tGFP
"BC020139" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204343 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGTGGTTGGCTCCGAGCTGCAGTCCGATGCTCAGCAGCTGAGCGCGGAAGCCCCGCAGCATGGAG AACTCCAGTACCTGAGGCAGGTGGAACACATTTTGCGCTGCGGCTTCAAGAAGGAGGACCGCACGGGCAC AGGCACCCTGTCGGTGTTCGGCATGCAGGCACGATACAGCCTGAGAGATGAATTTCCTCTGCTCACAACC AAACGAGTGTTCTGGAAGGGTGTTTTGGAGGAGTTGTTGTGGTTTATCAAGGGATCCACAAATGCTAAAG AATTGTCCTCCAAGGGAGTGAGAATCTGGGATGCCAATGGATCCCGAGATTTTCTGGACAGCTTGGGATT TTCTGCCCGACAGGAAGGGGACCTGGGCCCAGTTTATGGTTTCCAATGGAGGCATTTTGGAGCAGAGTAC AAAGATATGGATTCAGATTACTCGGGACAAGGAGTAGACCAGCTGCAAAAAGTGATTGACACCATCAAAA CCAACCCTGATGACAGAAGAATCATCATGTGTGCCTGGAACCCAAAAGATCTTCCCCTGATGGCACTGCC TCCTTGCCATGCCCTCTGTCAGTTCTATGTGGTGAATGGGGAACTGTCTTGCCAGCTTTACCAGAGGTCA GGAGATATGGGTCTGGGCGTGCCCTTCAACATTGCCAGCTATGCTCTGCTCACCTACATGATTGCACATA TCACAGGCCTGCAGCCAGGTGATTTTGTCCACACTTTGGGAGATGCACATATTTACCTGAATCATATAGA GCCGCTGAAAATTCAGCTACAGCGAGAACCAAGACCTTTCCCAAAGCTCAAAATCCTTCGAAAAGTTGAG ACAATCGATGATTTCAAAGTTGAAGACTTTCAGATTGAAGGGTATAATCCACATCCAACGATTAAAATGG AAATGGCTGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204343 protein sequence
Red=Cloning site Green=Tags(s) MLVVGSELQSDAQQLSAEAPQHGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLRDEFPLLTT KRVFWKGVLEELLWFIKGSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGAEY KDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRS GDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVE TIDDFKVEDFQIEGYNPHPTIKMEMAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC020139 |
| ORF Size | 921 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020139, AAH20139 |
| RefSeq Size | 986 bp |
| RefSeq ORF | 923 bp |
| Locus ID | 22171 |
| MW | 34.9 kDa |
| Gene Summary | This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407). The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon (PMID: 3444407, 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218262 | Tyms (untagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204), (10ug) |
CNY 6,940.00 |
|
| MG204343 | Tyms (tGFP-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 2,850.00 |
|
| MR204343L3 | Lenti ORF clone of Tyms (Myc-DDK-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 4,750.00 |
|
| MR204343L4 | Lenti ORF clone of Tyms (mGFP-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 4,750.00 |
