United States
Germany
Japan
United Kingdom
China
Fbxo6 (NM_001163704) Mouse Tagged ORF Clone
CAT＃: MR204081
- TrueORF®
Fbxo6 (Myc-DDK-tagged) - Mouse F-box protein 6 (Fbxo6), transcript variant 2
"NM_001163704" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA408845; FBG2; Fbs2; Fbx6b; Fbxo6b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204081 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCACATCAACGAGCTGCCAGAGAACATTCTCCTGGAGCTGTTCATCCATATCCCGGCCCCACAGC TGCTGCGCAACTGCCGCCTGGTCTGCCGCCTCTGGCGAGACCTCATCGATGTGGTGTCCCTATGGAAGCG CAAGAGTCTTCGAGAGGGCTTCTTCACCAAAGACCGGTGCGAGCCCGTGGAAGACTGGAAGGTCTTCTAT ATCCTGTGCAGCCTGCAGAGGAACCTCCTTCGGAACCCGTGTGCTGAAGAGAACCTGAGCTCATGGCGGA TAGACTCCAACGGAGGGGATCGCTGGAAGGTGGAGACGCTCCCTGGGAGCTGTGGCACAAGCTTTCCTGA CAACAAGGTCAAGAAGTATTTTGTCACCTCTTTTGAGATGTGCCTCAAATCCCAGATGGTGGACCTCAAA GCTGAGGGCTACTGCGAGGAGCTGATGGACACCTTTCGGCCTGACATTGTGGTTAAGGACTGGGTTGCCC CCAGAGCAGACTGTGGCTGCACCTATCAACTCCGGGTACAGCTGGCCTCTGCGGACTACATTGTCTTGGC CTCTTTTGAGCCTCCACCTGTGACATTCCAACAGTGGAATGATGCCAAATGGCAAGAGATTTCCCACACC TTCTCTGATTACCCTCCAGGTGTCCGTCACATCCTTTTTCAACACGGGGGCCAGGACACTCAGTTCTGGA AAGGCTGGTACGGCCCCCGTGTCACCAACAGCAGCATCATTATCAGCCACAGGACAGCCAAGAACCCTCC CCCTGCCAGAACTCTACCGGAAGAAACTGTAGTAATCGGAAGGAGACGGCGAGCTTCGGACTCCAACACT CATGAGGGTTTCTTCTGGCAAGGGCTATGGCAAAGGCTAAGGCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204081 protein sequence
Red=Cloning site Green=Tags(s) MVHINELPENILLELFIHIPAPQLLRNCRLVCRLWRDLIDVVSLWKRKSLREGFFTKDRCEPVEDWKVFY ILCSLQRNLLRNPCAEENLSSWRIDSNGGDRWKVETLPGSCGTSFPDNKVKKYFVTSFEMCLKSQMVDLK AEGYCEELMDTFRPDIVVKDWVAPRADCGCTYQLRVQLASADYIVLASFEPPPVTFQQWNDAKWQEISHT FSDYPPGVRHILFQHGGQDTQFWKGWYGPRVTNSSIIISHRTAKNPPPARTLPEETVVIGRRRRASDSNT HEGFFWQGLWQRLRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163704 |
| ORF Size | 888 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163704.1, NP_001157176.1 |
| RefSeq Size | 1429 bp |
| RefSeq ORF | 888 bp |
| Locus ID | 50762 |
| UniProt ID | Q9QZN4 |
| MW | 34.5 kDa |
| Gene Summary | Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication (By similarity). Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204081L3 | Lenti ORF clone of Fbxo6 (Myc-DDK-tagged) - Mouse F-box protein 6 (Fbxo6), transcript variant 2 |
CNY 4,750.00 |
|
| MR204081L4 | Lenti ORF clone of Fbxo6 (mGFP-tagged) - Mouse F-box protein 6 (Fbxo6), transcript variant 2 |
CNY 4,750.00 |
