United States
Germany
Japan
United Kingdom
China
Aptx (BC068309) Mouse Tagged ORF Clone
CAT＃: MR203707
- TrueORF®
Aptx (Myc-DDK-tagged) - Mouse aprataxin (cDNA clone MGC:76623 IMAGE:30102141)
ORF Plasmid: tGFP
"BC068309" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 2,760.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410016G21Rik; AA388047; FHA-HIT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203707 representing BC068309
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTCAACCCCACCAGCATTGACTCGGGCGTCATCGGGAAGGACCAAGAGAAGAAGCTGCTGCCTG GTCAGGTTCTCCACATGGTGAATGGACTTTATCCATACATCGTAGAGTTTGAGGAAGTGGCAGAGAGCCC TAACCTAACACAGAGGAAGAGAAAGAGGTCAGACTGTGATAGTGAGGAGATGGAAGCTGAGTCTGGGACA GGGCTGGCACCTGGGAGCAGCCCCAGCCAGTGCTCTGTGTCCCCTAAGAAGGACAAGAATGGAGCCACCA AAAAGGAATCACTGGGCCACTGGAGTCAAGGCTTGAAGATGTCTATGAAAGACCCCAAAATGCAGGTTTA CAAAGACGACCAGGTGGTGGTGATTAAGGATAAATACCCCAAGGCCCGTCACCACTGGCTGGTCTTACCG TGGGCCTCCATTTCCAGTCTGAAGGTTGTGACCAGTGAACACCTTGAACTTCTCAAACATATGCACGCTG TGGGGGAGAAGGTGATAGCAGATTTTGCTGGATCCAGCAAACTGCGCTTCCGATTGGGCTACCATGCCAT TCCCAGCATGAGCCACGTACATCTTCATGTGATCAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAA AAGCATTGGAATTCTTTTAATACAGAATACTTTCTGGAATCACAAGCTGTGATCAAGATGGTTCAGGAAG CCGGCAGAGTGACTGTTAAAGATGGCACTTGTGAGCTCTTGAAGCTGCCTCTCCGTTGCCATGAGTGTCA GCAGCTGCTGCCTTCCATCCCGCAGCTGAAAGAGCACCTCAGGAAGCACTGGGGCGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203707 representing BC068309
Red=Cloning site Green=Tags(s) MGVNPTSIDSGVIGKDQEKKLLPGQVLHMVNGLYPYIVEFEEVAESPNLTQRKRKRSDCDSEEMEAESGT GLAPGSSPSQCSVSPKKDKNGATKKESLGHWSQGLKMSMKDPKMQVYKDDQVVVIKDKYPKARHHWLVLP WASISSLKVVTSEHLELLKHMHAVGEKVIADFAGSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNK KHWNSFNTEYFLESQAVIKMVQEAGRVTVKDGTCELLKLPLRCHECQQLLPSIPQLKEHLRKHWGG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC068309 |
| ORF Size | 828 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068309 |
| RefSeq Size | 5171 bp |
| RefSeq ORF | 830 bp |
| Locus ID | 66408 |
| MW | 189.5 kDa |
| Gene Summary | DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (By similarity). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203707 | Aptx (tGFP-tagged) - Mouse aprataxin (cDNA clone MGC:76623 IMAGE:30102141) |
CNY 3,040.00 |
|
| MR203707L3 | Lenti ORF clone of Aptx (Myc-DDK-tagged) - Mouse aprataxin (cDNA clone MGC:76623 IMAGE:30102141) |
CNY 4,660.00 |
