Cela2a (NM_007919) Mouse Tagged Lenti ORF Clone
CAT#: MR203625L4
- LentiORF®
Lenti ORF clone of Cela2a (mGFP-tagged) - Mouse chymotrypsin-like elastase family, member 2A (Cela2a)
"NM_007919" in other vectors (4)
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CNY 5,890.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | El; Ela; Ela-2; Ela2; Ela2a |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR203625).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007919 |
ORF Size | 816 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007919.2 |
RefSeq Size | 912 bp |
RefSeq ORF | 816 bp |
Locus ID | 13706 |
UniProt ID | P05208 |
Gene Summary | This gene encodes a serine protease enzyme that hydrolyzes elastin. This gene is highly expressed in the pancreatic acinar cells where the encoded preproprotein undergoes processing including signal peptide cleavage to generate an inactive zymogen. The removal of N-terminal activation peptide from the zymogen by trypsin generates active elastase enzyme. This gene is also expressed in the mouse epidermis where it participates in pro-filaggrin processing. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201774 | Cela2a (untagged) - Mouse chymotrypsin-like elastase family, member 2A (Cela2a), (10ug) |
CNY 3,600.00 |
|
MG203625 | Cela2a (tGFP-tagged) - Mouse elastase 2A (Ela2a) |
CNY 5,200.00 |
|
MR203625 | Cela2a (Myc-DDK-tagged) - Mouse chymotrypsin-like elastase family, member 2A (Cela2a) |
CNY 3,600.00 |
|
MR203625L3 | Lenti ORF clone of Cela2a (Myc-DDK-tagged) - Mouse chymotrypsin-like elastase family, member 2A (Cela2a) |
CNY 5,890.00 |