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Il33 (NM_001164724) Mouse Tagged ORF Clone
CAT＃: MR203528
- TrueORF®
Il33 (Myc-DDK-tagged) - Mouse interleukin 33 (Il33), transcript variant 1
"NM_001164724" in other vectors (2)
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CNY 1,416.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9230117N10Rik; Il-33; Il1f11; NF-HEV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203528 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACCTAGAATGAAGTATTCCAACTCCAAGATTTCCCCGGCAAAGTTCAGCAGCACCGCAGGCGAAG CCCTGGTCCCGCCTTGCAAAATAAGAAGATCCCAACAGAAGACCAAAGAATTCTGCCATGTCTACTGCAT GAGACTCCGTTCTGGCCTCACCATAAGAAAGGAGACTAGTTATTTTAGGAAAGAACCCACGAAAAGATAT TCACTAAAATCGGGTACCAAGCATGAAGAGAACTTCTCTGCCTATCCACGGGATTCTAGGAAGAGATCCT TGCTTGGCAGTATCCAAGCATTTGCTGCGTCTGTTGACACATTGAGCATCCAAGGAACTTCACTTTTAAC ACAGTCTCCTGCCTCCCTGAGTACATACAATGACCAATCTGTTAGTTTTGTTTTGGAGAATGGATGTTAT GTGATCAATGTTGACGACTCTGGAAAAGACCAAGAGCAAGACCAGGTGCTACTACGCTACTATGAGTCTC CCTGTCCTGCAAGTCAATCAGGCGACGGTGTGGATGGGAAGAAGGTGATGGTGAACATGAGTCCCATCAA AGACACAGACATCTGGCTGCATGCCAACGACAAGGACTACTCCGTGGAGCTTCAAAGGGGTGACGTCTCG CCTCCGGAACAGGCCTTCTTCGTCCTTCACAAAAAGTCCTCGGACTTTGTTTCATTTGAATGCAAGAATC TTCCTGGCACTTACATAGGAGTAAAAGATAACCAGCTGGCTCTAGTGGAGGAGAAAGATGAGAGCTGCAA CAATATTATGTTTAAGCTCTCGAAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203528 protein sequence
Red=Cloning site Green=Tags(s) MRPRMKYSNSKISPAKFSSTAGEALVPPCKIRRSQQKTKEFCHVYCMRLRSGLTIRKETSYFRKEPTKRY SLKSGTKHEENFSAYPRDSRKRSLLGSIQAFAASVDTLSIQGTSLLTQSPASLSTYNDQSVSFVLENGCY VINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKVMVNMSPIKDTDIWLHANDKDYSVELQRGDVS PPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164724 |
| ORF Size | 801 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164724.1, NP_001158196.1 |
| RefSeq Size | 2555 bp |
| RefSeq ORF | 801 bp |
| Locus ID | 77125 |
| UniProt ID | Q8BVZ5 |
| MW | 30 kDa |
| Gene Summary | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Aberrant hyperexpression of the RNA binding protein FMRP in tumors mediates immune evasion.
,null,
Science (New York, N.Y.)
,PubMed ID 36395212
[Il33]
|
|
IL33 is a key driver of treatment resistance of cancer
,Kudo-Saito, C;Miyamoto, T;Imazeki, H;Shoji, H;Aoki, K;Boku, N;,
Cancer Res.
,PubMed ID 32156776
[IL33]
|
|
Notch Inhibition Enhances Cardiac Reprogramming by Increasing MEF2C Transcriptional Activity
,Abad, M;Hashimoto, H;Zhou, H;Morales, MG;Chen, B;Bassel-Duby, R;Olson, EN;,
Stem Cell Reports
,PubMed ID 28262548
[IL33]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203528L3 | Lenti ORF clone of Il33 (Myc-DDK-tagged) - Mouse interleukin 33 (Il33), transcript variant 1 |
CNY 6,000.00 |
|
| MR203528L4 | Lenti ORF clone of Il33 (mGFP-tagged) - Mouse interleukin 33 (Il33), transcript variant 1 |
CNY 6,000.00 |
