Cavin3 (NM_028444) Mouse Tagged ORF Clone

CAT#: MR203388

  • TrueORF®

Prkcdbp (Myc-DDK-tagged) - Mouse protein kinase C, delta binding protein (Prkcdbp)

ORF Plasmid: DDK tGFP



  "NM_028444" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-Prkcdbp Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 3110015B12Rik; 6330514M23Rik; SRBC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203388 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGAGCGCACTGGAGCCCGGACCTGTGCCTGAGACGCCCGCAGGGGGTCCGGTGCACGCAGTCA
CCGTGGTGACCTTGCTGGAGAAATTGGCCACCATGCTGGAGGCTCTGCGGGAGCGGCAAGGCGGCCTGGC
TGAGAGGCAGGGCGGCCTGGCGGGCTCCGTGCGTCGCATACAGAGTGGCCTGGGTGCGCTGAGTCGCAGC
CACGACACCACCAGCAACACACTGACGCAGCTGCTGGCCAAGGCGGAGCGCGTGGGCTCGCACGCCGACG
CAGCCCAGGAGCGGGCCGTGCGTCGCGCTGCTCAGGTGCAGCGGTTGGAGGCCAACCACGGGCTGCTGGT
GGCGCGCGGGAAGCTGCACGTCCTGCTCTTCAAGGAGGAGACTGAAATTCCAGCCCGCGCCTTCCAGAAA
GTCCCAGAGCTCTTGGGTCCGGAGGACCAGTTGGTGCTGGGCCCAGATCAGCCAGAGGATGAAGTTGGAG
AGAGTTCTGAAGAGGAGCCAGTGGAGTCCAGGGCTCAGCGGCTGCGCCGCACTGGTTTACAGAAAGTTCA
AAGCCTGAAAAGGGCTCTTTCCAGTCGTAAAGCAGCACAGCCCACACCAGTCAAGCCGCCACGCGTAGGG
CCTGTCCGGAGCTCTGAAGGCCCATCAGAAGGCCAGCCTGCAGCTCAGCCTGAAATGGAGTCCGAGCTCG
AGACTGCCCTGGAGCCAGAACCTCCTCAACCTACCAAGGAAGATCCTGAGAAACCCGTGCTTCAAATAGA
GAGCGCAGCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR203388 protein sequence
Red=Cloning site Green=Tags(s)

MGESALEPGPVPETPAGGPVHAVTVVTLLEKLATMLEALRERQGGLAERQGGLAGSVRRIQSGLGALSRS
HDTTSNTLTQLLAKAERVGSHADAAQERAVRRAAQVQRLEANHGLLVARGKLHVLLFKEETEIPARAFQK
VPELLGPEDQLVLGPDQPEDEVGESSEEEPVESRAQRLRRTGLQKVQSLKRALSSRKAAQPTPVKPPRVG
PVRSSEGPSEGQPAAQPEMESELETALEPEPPQPTKEDPEKPVLQIESAA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028444
ORF Size 783 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_028444.1, NP_082720.1
RefSeq Size 1026 bp
RefSeq ORF 783 bp
Locus ID 109042
UniProt ID Q91VJ2
MW 27.9 kDa
Gene Summary Regulates the traffic and/or budding of caveolae (By similarity). Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in smooth muscle but not in the lung and heart endothelial cells (PubMed: 28285351, PubMed:23652019). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by promoting the rapid release of caveolae from the cell surface (PubMed:25588833). Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2 (PubMed:23079727).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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