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Mdfi (NM_010783) Mouse Tagged Lenti ORF Clone
CAT＃: MR203079L2
- LentiORF®
Lenti ORF clone of Mdfi (mGFP-tagged) - Mouse MyoD family inhibitor (Mdfi), transcript variant 1
"NM_010783" in other vectors (4)
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CNY 6,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | I-mf; I-mfa |
| Vector | pLenti-C-mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR203079).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010783 |
| ORF Size | 741 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010783.2 |
| RefSeq Size | 1379 bp |
| RefSeq ORF | 741 bp |
| Locus ID | 17240 |
| UniProt ID | P70331 |
| Gene Summary | Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203079 | Mdfi (Myc-DDK-tagged) - Mouse MyoD family inhibitor (Mdfi), transcript variant 1 |
CNY 3,600.00 |
|
| MR203079L1 | Lenti ORF clone of Mdfi (Myc-DDK-tagged) - Mouse MyoD family inhibitor (Mdfi), transcript variant 1 |
CNY 4,370.00 |
|
| MR203079L3 | Lenti ORF clone of Mdfi (Myc-DDK-tagged) - Mouse MyoD family inhibitor (Mdfi), transcript variant 1 |
CNY 4,370.00 |
|
| MR203079L4 | Lenti ORF clone of Mdfi (mGFP-tagged) - Mouse MyoD family inhibitor (Mdfi), transcript variant 1 |
CNY 4,370.00 |
