Otub2 (NM_026580) Mouse Tagged ORF Clone
CAT#: MR202832
- TrueORF®
Otub2 (Myc-DDK-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2
ORF Plasmid: tGFP
"NM_026580" in other vectors (4)
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CNY 3,600.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2010015L18Rik; 4930586I02Rik; AI413508; AW557219; OTB2; OTU2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202832 representing NM_026580
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAACATCTTTCAACCTAATATCAGAAAAATGTGACATTCTATCCATTCTTCGGGATCATCCTG AAAACAGGATTTACCAGAGGAAAATCCAGGAACTCAGCAAAAGATTCACCTCGATCCGGAAGACCAAAGG AGACGGAAACTGCTTCTACAGGGCCTTAGGCTATTCCTACCTGGAGTCCTTGCTGGGCAAGAGCAGAGAG ATCCTCAAGTTCAAAGAGCGTGTGCTACAGACCCCAAATGACCTTCTGGCTGCCGGCTTTGAGGAACACA AGTTCAGAAACTTCTTTAATGCTTTTTACAGTGTGGTTGAGCTGGTAGAGAAGGATAGCTCAGTGTCCAG CCTGCTGAAGGTGTTCAATGACCAGAGTTCCTCGGACCGAATCGTGCAGTTCTTACGCCTCCTCACGTCG GCCTTCATCAGGAACCGAGCTGACTTCTTCCGACATTTCATTGATGAGGAGATGGACATCAAAGACTTCT GCACTCACGAAGTAGAGCCCATGGCCATGGAGTGTGACCACGTGCAGATCACAGCCCTGTCGCAGGCACT CAACATTGCTCTGCAGGTAGAGTACGTCGACGAGATGGACACCGCTCTGAACCACCACGTGTTCCCCGAG GCTGCCATCCCTTCGGTTTATCTGCTCTATAAAACATCCCACTACAACATCCTTTACGCAGCCGAGAAAC AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202832 representing NM_026580
Red=Cloning site Green=Tags(s) MSETSFNLISEKCDILSILRDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSRE ILKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDSSVSSLLKVFNDQSSSDRIVQFLRLLTS AFIRNRADFFRHFIDEEMDIKDFCTHEVEPMAMECDHVQITALSQALNIALQVEYVDEMDTALNHHVFPE AAIPSVYLLYKTSHYNILYAAEKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026580 |
ORF Size | 702 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026580.4, NP_080856.1 |
RefSeq Size | 2813 bp |
RefSeq ORF | 705 bp |
Locus ID | 68149 |
UniProt ID | Q9CQX0 |
MW | 27.8 kDa |
Gene Summary | Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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OTUD1 Regulates Antifungal Innate Immunity through Deubiquitination of CARD9
,Chen, X;Zhang, H;Wang, X;Shao, Z;Li, Y;Zhao, G;Liu, F;Liu, B;Zheng, Y;Chen, T;Zheng, H;Zhang, L;Gao, C;,
Journal of immunology (Baltimore, Md. : 1950) 2021
,PubMed ID 33789983
[OTUB2]
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Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204918 | Otub2 (untagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2, (10ug) |
CNY 3,600.00 |
|
MG202832 | Otub2 (tGFP-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2) |
CNY 5,200.00 |
|
MR202832L3 | Lenti ORF clone of Otub2 (Myc-DDK-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2 |
CNY 5,890.00 |
|
MR202832L4 | Lenti ORF clone of Otub2 (mGFP-tagged) - Mouse OTU domain, ubiquitin aldehyde binding 2 (Otub2), transcript variant 2 |
CNY 5,890.00 |