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Cltb (BC070404) Mouse Tagged ORF Clone
CAT＃: MR202747
- TrueORF®
Cltb (Myc-DDK-tagged) - Mouse clathrin, light polypeptide (Lcb) (cDNA clone MGC:92950 IMAGE:5699653)
ORF Plasmid: tGFP
"BC070404" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310046E19Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202747 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGGACTTCGGCTTCTTCTCATCGTCGGAGAGCGGGGCCCCGGAGGCCGCCGAGGAGGACCCAG CGGCCGCCTTCCTGGCCCAGCAGGAAAGCGAGATTGCTGGCATCGAGAATGACCCGGGTTTCGGGGCACC TGCCGCCAGCCAGGTGGCCTCTGCGCAGCCGGGACTCGCGAGCGGGGCTGGTTCAGAGGACATGAGTACT ACAGTCAATGGAGATGTGTTTCAGGAGGCTAACGGGCCTGCCGATGGCTACGCTGCGATTGCCCAGGCGG ACAGGTTGACTCAGGAGCCCGAGAGCATCCGGAAGTGGAGAGAGGAGCAGAAGAAAAGGCTGCAGGAGTT AGACGCTGCCTCGAAGGTGACCGAACAGGAGTGGCGGGAGAAGGCCAAGAAAGACCTGGAGGAGTGGAAC CAGCGCCAAAGTGAACAGGTTGAGAAGAACAAGATCAACAACAGGATCGCTGACAAAGCGTTCTACCAGC AGCCAGATGCTGATACCATTGGCTATGTGGCATCGGAAGAGGCTTTTGTGAAAGAATCCAAGGAGGAGAC CCCGGGCACAGAGTGGGAGAAGGTAGCCCAGCTGTGTGACTTCAATCCCAAGAGCAGCAAGCAGTGTAAA GATGTGTCCCGCCTGCGCTCGGTGCTCATGTCCCTGAAGCAAACACCACTGTCCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202747 protein sequence
Red=Cloning site Green=Tags(s) MAEDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDPGFGAPAASQVASAQPGLASGAGSEDMST TVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQKKRLQELDAASKVTEQEWREKAKKDLEEWN QRQSEQVEKNKINNRIADKAFYQQPDADTIGYVASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCK DVSRLRSVLMSLKQTPLSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC070404 |
| ORF Size | 687 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC070404, AAH70404 |
| RefSeq Size | 1009 bp |
| RefSeq ORF | 689 bp |
| Locus ID | 74325 |
| MW | 25.2 kDa |
| Gene Summary | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217907 | Cltb (untagged) - Mouse clathrin, light polypeptide (Lcb) (cDNA clone MGC:92950 IMAGE:5699653), (10ug) |
CNY 6,940.00 |
|
| MG202747 | Cltb (tGFP-tagged) - Mouse clathrin, light polypeptide (Lcb) (cDNA clone MGC:92950 IMAGE:5699653) |
CNY 2,850.00 |
|
| MR202747L3 | Lenti ORF clone of Cltb (Myc-DDK-tagged) - Mouse clathrin, light polypeptide (Lcb) (cDNA clone MGC:92950 IMAGE:5699653) |
CNY 4,750.00 |
|
| MR202747L4 | Lenti ORF clone of Cltb (mGFP-tagged) - Mouse clathrin, light polypeptide (Lcb) (cDNA clone MGC:92950 IMAGE:5699653) |
CNY 4,750.00 |
