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Ntmt1 (NM_170592) Mouse Tagged ORF Clone
CAT＃: MR202578
- TrueORF®
Ntmt1 (Myc-DDK-tagged) - Mouse methyltransferase like 11A (Mettl11a)
ORF Plasmid: tGFP
"NM_170592" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610205E22Rik; AL033331; AL033332; Mettl11a; NTM1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGAGCGAGGTGATTGAGGATGAGAAACAGTTTTATTCGAAGGCCAAGACCTACTGGAAGCAGATCC CACCCACGGTGGACGGCATGCTCGGGGGGTATGGCCACATCTCCAACATCGACCTCAACAGCTCCCGGAA GTTTCTACAGAGGTTTTTAAGGGAAGGCCCAAACAAGACAGGGACTTCCTGTGCCTTGGACTGTGGCGCT GGTATTGGAAGGATCACCAAGCGCCTGCTCCTGCCACTCTTCAGGGTGGTCGACATGGTCGACGTGACAG AAGACTTTCTGGCCAAAGCCAAGACCTACCTGGGGGAAGAGGGCAAGAGGGTGAGGAACTACTTCTGCTG TGGGCTGCAGGACTTCAGCCCTGAGCCTGGCTCCTATGACGTGATCTGGATCCAGTGGGTGATAGGCCAC CTGACGGATCAGCACCTGGCTGAGTTTCTGCGCCGCTGCAAGCGGGGCCTGCGCCCCAATGGCATCATCG TCATCAAGGACAACATGGCCCAGGAGGGTGTGATCCTGGACGATGTGGACAGCAGTGTGTGCCGGGACCT TGAGGTGGTCCGCCGGATCATCCGCACTGCAGGCCTCAGCCTCCTGGCCGAGGAGCGCCAGGAGAACCTG CCAGATGAGATCTACCATGTCTACAGCTTTGCCCTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202578 protein sequence
Red=Cloning site Green=Tags(s) MTSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISNIDLNSSRKFLQRFLREGPNKTGTSCALDCGA GIGRITKRLLLPLFRVVDMVDVTEDFLAKAKTYLGEEGKRVRNYFCCGLQDFSPEPGSYDVIWIQWVIGH LTDQHLAEFLRRCKRGLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLEVVRRIIRTAGLSLLAEERQENL PDEIYHVYSFALR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_170592 |
| ORF Size | 672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_170592.1 |
| RefSeq Size | 1217 bp |
| RefSeq ORF | 672 bp |
| Locus ID | 66617 |
| UniProt ID | Q8R2U4 |
| MW | 25.4 kDa |
| Gene Summary | Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif (PubMed:20668449). Some of the substrates may be primed by METTL11B-mediated monomethylation. Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1) (By similarity). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1 (PubMed:20668449).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202863 | Ntmt1 (untagged) - Mouse methyltransferase like 11A (Mettl11a), (10ug) |
CNY 2,400.00 |
|
| MG202578 | Ntmt1 (tGFP-tagged) - Mouse RIKEN cDNA 2610205E22 gene (2610205E22Rik) |
CNY 2,850.00 |
|
| MR202578L3 | Lenti ORF clone of Ntmt1 (Myc-DDK-tagged) - Mouse methyltransferase like 11A (Mettl11a) |
CNY 4,750.00 |
|
| MR202578L4 | Lenti ORF clone of Ntmt1 (mGFP-tagged) - Mouse methyltransferase like 11A (Mettl11a) |
CNY 4,750.00 |
