Rab21 (NM_024454) Mouse Tagged ORF Clone

CAT#: MR202564

  • TrueORF®

Rab21 (Myc-DDK-tagged) - Mouse RAB21, member RAS oncogene family (Rab21)

ORF Plasmid: DDK tGFP



  "NM_024454" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,081.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 9630024B22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202564 representing NM_024454
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGCCGGCGGCGGGGCGGCGGCGGCGGCGGGCCGAGCCTACTCCTTCAAGGTGGTGCTGCTCG
GGGAAGGCTGCGTGGGGAAGACGTCGCTGGTGCTGCGCTACTGCGAGAACAAGTTCAACGACAAGCACAT
CACCACCCTGCAGGCATCTTTCTTAACAAAGAAGTTAAATATTGGTGGAAAAAGAGTAAACCTTGCCATA
TGGGACACAGCAGGTCAGGAGAGATTCCACGCACTGGGCCCAATTTACTACCGAGATTCGAACGGAGCTA
TTTTAGTTTATGATGTAACGGATGAAGATTCCTTTCAGAAGGTAAAAAACTGGGTCAAAGAATTACGAAA
AATGTTGGGAAATGAGATCTGTTTGTGTATAGTTGGTAATAAAATAGACTTGGAAAAGGAGAGGCACGTC
TCTATTCAAGAAGCAGAATCGTATGCAGAGTCTGTGGGAGCCAAGCATTACCATACTTCCGCTAAACAGA
ACAAAGGCATTGAGGAGCTCTTTCTCGACCTTTGTAAAAGAATGATAGAGACAGCCCAGGTGGATGAGAG
AGCAAAAGGCAATGGCTCGAGTCAAGCCGGGGCTGCAAGGCGAGGTGTGCAGATCATCGACGACGAGCCT
CAAGCTCAGAGCAGCGGAGGGTGCTGTTCCTCCGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202564 representing NM_024454
Red=Cloning site Green=Tags(s)

MAAAGGGAAAAAGRAYSFKVVLLGEGCVGKTSLVLRYCENKFNDKHITTLQASFLTKKLNIGGKRVNLAI
WDTAGQERFHALGPIYYRDSNGAILVYDVTDEDSFQKVKNWVKELRKMLGNEICLCIVGNKIDLEKERHV
SIQEAESYAESVGAKHYHTSAKQNKGIEELFLDLCKRMIETAQVDERAKGNGSSQAGAARRGVQIIDDEP
QAQSSGGCCSSG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024454
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024454.1, NP_077774.1
RefSeq Size 1856 bp
RefSeq ORF 669 bp
Locus ID 216344
UniProt ID P35282
MW 24.6 kDa
Gene Summary Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general (PubMed:16754960). As a result, may regulate cell adhesion and migration (PubMed:16754960). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis (PubMed:18804435). Involved in neurite growth (By similarity). Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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