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Dkk4 (NM_145592) Mouse Tagged ORF Clone
CAT＃: MR202533
- TrueORF®
Dkk4 (Myc-DDK-tagged) - Mouse dickkopf homolog 4 (Xenopus laevis) (Dkk4)
ORF Plasmid: tGFP
"NM_145592" in other vectors (4)
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CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dkk-4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202533 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACTGGTGACCTTGCTTGGACTCAGCTGGTTTTGTTCACCCCTGGCAGCCCTGGTTCTGGACTTTA ACAACATCAAGAGCTCCGCGGATGTGCAAGGCGCGGGGAAGGGCTCGCTGTGTGCATCAGACAGGGACTG CAGCGAAGGGAAATTCTGCTTAGCGTTTCACGATGAACGGTCGTTCTGTGCCACGTGCCGTAGAGTTCGC AGGAGGTGTCAGAGGAGCGCCGTGTGCTGCCCAGGAACGGTCTGTGTGAATGATGTTTGCACTGCAGTGG AAGACACAAGGCCAGTGATGGACAGAAACACTGACGGCCAAGACGGCGCCTATGCAGAAGGAACCACTAA ATGGCCAGCAGAGGAAAACAGACCTCAGGGGAAGCCCAGTACGAAGAAATCACAAAGCAGTAAGGGACAG GAGGGAGAAAGCTGTCTTAGAACCTCTGACTGTGGCCCTGGACTTTGCTGTGCTCGCCATTTTTGGACAA AAATTTGCAAGCCAGTTCTACGAGAGGGACAAGTCTGCTCCAGGAGGGGGCACAAAGACACTGCCCAAGC CCCAGAAATCTTCCAGCGTTGCGACTGCGGGCCTGGACTAACGTGCCGAAGTCAGGTGACCAGTAACAGA CAACATTCAAGGCTAAGAGTATGCCAAAGAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202533 protein sequence
Red=Cloning site Green=Tags(s) MVLVTLLGLSWFCSPLAALVLDFNNIKSSADVQGAGKGSLCASDRDCSEGKFCLAFHDERSFCATCRRVR RRCQRSAVCCPGTVCVNDVCTAVEDTRPVMDRNTDGQDGAYAEGTTKWPAEENRPQGKPSTKKSQSSKGQ EGESCLRTSDCGPGLCCARHFWTKICKPVLREGQVCSRRGHKDTAQAPEIFQRCDCGPGLTCRSQVTSNR QHSRLRVCQRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145592 |
| ORF Size | 666 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145592.1 |
| RefSeq Size | 1315 bp |
| RefSeq ORF | 666 bp |
| Locus ID | 234130 |
| UniProt ID | Q8VEJ3 |
| MW | 24.3 kDa |
| Gene Summary | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Progenitors oppositely polarize WNT activators and inhibitors to orchestrate tissue development
,null,
eLife
,PubMed ID 32310087
[Dkk4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201440 | Dkk4 (untagged) - Mouse dickkopf homolog 4 (Xenopus laevis) (Dkk4), (10ug) |
CNY 3,600.00 |
|
| MG202533 | Dkk4 (tGFP-tagged) - Mouse dickkopf homolog 4 (Xenopus laevis) (Dkk4) |
CNY 5,200.00 |
|
| MR202533L3 | Lenti ORF clone of Dkk4 (Myc-DDK-tagged) - Mouse dickkopf homolog 4 (Xenopus laevis) (Dkk4) |
CNY 5,890.00 |
|
| MR202533L4 | Lenti ORF clone of Dkk4 (mGFP-tagged) - Mouse dickkopf homolog 4 (Xenopus laevis) (Dkk4) |
CNY 5,890.00 |
