Ak1 (NM_021515) Mouse Tagged ORF Clone
CAT#: MR202255
- TrueORF®
Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (Ak1), transcript variant 1
ORF Plasmid: tGFP
"NM_021515" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Ak-1; B430205N08Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202255 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCTGTGTGTCTAGTGAACCCCAAGAAGAGGGAGGCAGAAAGACTGGAGAGAAGCTGAAGAAGG CCAAGATCATCTTTGTGGTGGGCGGACCTGGCTCAGGAAAGGGCACCCAGTGCGAGAAGATTGTACAGAA ATATGGCTACACCCACCTGTCTACTGGGGACCTGCTTCGGGCAGAAGTCAGCTCTGGATCGGAGAGGGGC AAGAAGCTGTCGGCTATCATGGAGAAGGGAGAGCTAGTGCCACTGGACACGGTGCTGGACATGCTCCGAG ATGCTATGTTAGCCAAAGTGGATTCTTCCAATGGCTTCCTGATCGACGGCTACCCGAGGGAGGTGAAACA GGGAGAAGAATTTGAACAGAAGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCGCCGAGACC ATGACCCAACGACTCCTGAAGCGAGGGGAGACCAGTGGCCGCGTGGATGACAACGAGGAGACCATCAAGA AGCGGCTGGAGACTTATTACAATGCCACAGAACCTGTCATCTCCTTCTATGACAAGCGTGGCATTGTGCG CAAGGTCAATGCCGAAGGCACGGTGGACACTGTCTTCTCTGAGGTCTGCACCTATCTTGACTCCCTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202255 protein sequence
Red=Cloning site Green=Tags(s) MGCCVSSEPQEEGGRKTGEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERG KKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAET MTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021515 |
ORF Size | 633 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021515.3 |
RefSeq Size | 2065 bp |
RefSeq ORF | 633 bp |
Locus ID | 11636 |
UniProt ID | Q9R0Y5 |
MW | 23.1 kDa |
Gene Summary | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity). May provide a mechanism to buffer the adenylate energy charge for sperm motility.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202285 | Ak1 (untagged) - Mouse adenylate kinase 1 (Ak1), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
MG202255 | Ak1 (tGFP-tagged) - Mouse adenylate kinase 1 (Ak1) |
CNY 2,850.00 |
|
MR202255L3 | Lenti ORF clone of Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (Ak1), transcript variant 1 |
CNY 4,750.00 |
|
MR202255L4 | Lenti ORF clone of Ak1 (mGFP-tagged) - Mouse adenylate kinase 1 (Ak1), transcript variant 1 |
CNY 4,750.00 |