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Josd1 (NM_028792) Mouse Tagged ORF Clone
CAT＃: MR202076
- TrueORF®
Josd1 (Myc-DDK-tagged) - Mouse Josephin domain containing 1 (Josd1)
ORF Plasmid: tGFP
"NM_028792" in other vectors (4)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300006C06Rik; mKIAA0063 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202076 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTGCGTGCCATGGAAAGGAGACAAGGCCAAAGCTGAATCCTCGGACCTTCCCCAAGCAGCGCCCC CACAAATCTACCATGAGAAGCAGCGCAGGGAGCTCTGTGCTCTTCATGCTCTCAACAACGTCTTCCAGGA CAGCAACGCCTTCACGCGGGAAACGCTGCAGGAGATTTTCCAGAGGCTGTCTCCGAACACTATGGTGACG CCCCACAAGAAGAGCATGCTGGGAAATGGGAACTATGATGTGAATGTCATCATGGCAGCACTTCAAACCA AAGGCTATGAAGCTGTTTGGTGGGACAAGCGAAGGGACGTGGGTGTCATTGCTCTTACTAATGTCATGGG CTTCATCATGAACCTGCCCTCCAGCCTCTGCTGGGGTCCACTCAAGTTGCCTCTCAAAAGACAGCACTGG ATCTGTGTTCGTGAGGTGGGAGGGGCCTACTACAACCTTGACTCCAAACTCAAGATGCCAGAGTGGATTG GAGGCGAGAGTGAGCTCAGGAAATTTCTAAAATACCATTTGCGAGGTAAAAACTGTGAACTCCTACTGGT TGTACCGGAAGAAGTGGAAGCCCATCAGAGCTGGAGGGCCGACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202076 protein sequence
Red=Cloning site Green=Tags(s) MSCVPWKGDKAKAESSDLPQAAPPQIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRLSPNTMVT PHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIMNLPSSLCWGPLKLPLKRQHW ICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGKNCELLLVVPEEVEAHQSWRADV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028792 |
| ORF Size | 609 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028792.3, NP_083068.1 |
| RefSeq Size | 3394 bp |
| RefSeq ORF | 609 bp |
| Locus ID | 74158 |
| UniProt ID | Q9DBJ6 |
| MW | 23.2 kDa |
| Gene Summary | Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207738 | Josd1 (untagged) - Mouse Josephin domain containing 1 (Josd1), (10ug) |
CNY 3,990.00 |
|
| MG202076 | Josd1 (tGFP-tagged) - Mouse Josephin domain containing 1 (Josd1) |
CNY 2,850.00 |
|
| MR202076L3 | Lenti ORF clone of Josd1 (Myc-DDK-tagged) - Mouse Josephin domain containing 1 (Josd1) |
CNY 4,750.00 |
|
| MR202076L4 | Lenti ORF clone of Josd1 (mGFP-tagged) - Mouse Josephin domain containing 1 (Josd1) |
CNY 4,750.00 |
