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Rbpms (NM_019733) Mouse Tagged ORF Clone
CAT＃: MR201952
- TrueORF®
Rbpms (Myc-DDK-tagged) - Mouse RNA binding protein gene with multiple splicing (Rbpms), transcript variant 1
"NM_019733" in other vectors (3)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010300K22Rik; 2700019M19Rik; AU017537; hermes; RBP-MS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201952 representing NM_019733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGCGGCGGCAAAGCCGAGAAGGAGAACACCCCGAGCGAGGCCAACCTTCAGGAGGAGGAGGTCC GGACCCTATTTGTCAGCGGTCTGCCTCTGGACATCAAGCCCCGAGAGCTGTACCTGCTCTTCAGACCATT CAAGGGCTATGAAGGTTCTCTCATAAAGCTCACATCTAAACAGCCCGTAGGCTTTGTCAGTTTTGACAGT CGCTCAGAAGCAGAGGCCGCAAAGAACGCTTTGAACGGCATCCGCTTCGATCCTGAAATCCCGCAAACGC TACGACTAGAGTTTGCTAAGGCAAACACGAAGATGGCCAAGAACAAACTCGTAGGGACTCCAAACCCCAG TACTCCTCTGCCCAACACTGTACCTCAGTTCATTGCCAGGGAGCCATATGAGCTCACAGTACCTGCACTT TACCCCAGTAGCCCTGAAGTTTGGGCCCCGTACCCTCTGTACCCAGCGGAGTTAGCGCCTGCTCTTCCTC CTCCTGCCGCTTTCACCTACCCCGCTTCACTGCATGCCCAGATGCGCTGGATCCCTCCCTCCGAGGCCAC TTCCCAGGGCTGGAAGTCCCGGCAGTTCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201952 representing NM_019733
Red=Cloning site Green=Tags(s) MNGGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDS RSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPAL YPSSPEVWAPYPLYPAELAPALPPPAAFTYPASLHAQMRWIPPSEATSQGWKSRQFC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019733 |
| ORF Size | 591 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019733.3, NP_062707.1 |
| RefSeq Size | 2569 bp |
| RefSeq ORF | 594 bp |
| Locus ID | 19663 |
| UniProt ID | Q9WVB0 |
| MW | 22.3 kDa |
| Gene Summary | Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209306 | Rbpms (untagged) - Mouse RNA binding protein gene with multiple splicing (Rbpms), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR201952L3 | Lenti ORF clone of Rbpms (Myc-DDK-tagged) - Mouse RNA binding protein gene with multiple splicing (Rbpms), transcript variant 1 |
CNY 4,750.00 |
|
| MR201952L4 | Lenti ORF clone of Rbpms (mGFP-tagged) - Mouse RNA binding protein gene with multiple splicing (Rbpms), transcript variant 1 |
CNY 4,750.00 |
