Ssu72 (BC016544) Mouse Tagged ORF Clone

CAT#: MR201644

  • TrueORF®

Ssu72 (Myc-DDK-tagged) - Mouse Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) (cDNA clone MGC:27889 IMAGE:3497878)

ORF Plasmid: DDK tGFP



  "BC016544" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 2,945.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1190002E22Rik; 1500011L16Rik; 2610101M12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201644 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTCATCGCCGCTGCGGGTGGCGGTGGTGTGCTCGAGTAACCAGAATCGGAGCATGGAAGCACACA
ACATCCTCAGCAAGCGAGGTTTCAGTGTCCGCTCCTTTGGAACAGGAACTCATGTGAAGCTCCCAGGACC
AGCACCTGACAAGCCCAATGTTTATGACTTCAAAACCACATATGACCAGATGTACAATGACCTCCTCAGG
AAAGACAAAGAACTCTATACACAAAATGGAATTTTACATATGTTGGACAGAAATAAAAGGATCAAGCCCC
GTCCAGAAAGGTTCCAGAACTGCACGGACCTGTTTGACTTGATCCTCACTTGTGAAGAGAGAGTCTATGA
CCAGGTTGTAGAAGATCTGAACTCCAGAGAACAGGAGACCTGCCAGCCAGTGCATGTGGTCAATGTGGAC
ATCCAGGACAACCATGAAGAGGCCACTCTGGGAGCATTCCTTATCTGCGAGCTCTGCCAGTGTGTGAGTC
TAAGCTCCTGGGTCCTGTTGGGGCTTCTCATAGCAACTTATAAAAATAAAATAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201644 protein sequence
Red=Cloning site Green=Tags(s)

MPSSPLRVAVVCSSNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTYDQMYNDLLR
KDKELYTQNGILHMLDRNKRIKPRPERFQNCTDLFDLILTCEERVYDQVVEDLNSREQETCQPVHVVNVD
IQDNHEEATLGAFLICELCQCVSLSSWVLLGLLIATYKNKIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC016544
ORF Size 546 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC016544, AAH16544
RefSeq Size 884 bp
RefSeq ORF 548 bp
Locus ID 68991
MW 20.9 kDa
Gene Summary Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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