Myl1 (BC059087) Mouse Tagged ORF Clone
CAT#: MR201424
- TrueORF®
Myl1 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 1 (cDNA clone MGC:70067 IMAGE:30139109)
ORF Plasmid: tGFP
"BC059087" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 1,900.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MLC1f, MLC3f |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201424 representing BC059087
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTCAGTGCTGACCAGATTGCCGACTTCAAGGAGGCATTTCTCCTGTTTGACAGAACAGGTGAAT GCAAGATCACCTTAAGTCAGGTGGGAGACGTCCTCCGGGCTCTGGGCACCAATCCCACCAATGCAGAGGT CAAGAAGGTTCTTGGGAACCCCAGCAATGAAGAGATGAATGCTAAGAAAATCGAGTTTGAACAGTTTCTG CCCATGATGCAAGCTATCTCCAACAACAAGGACCAGGGAGGTTATGAAGATTTCGTTGAGGGTCTGCGTG TCTTCGACAAGGAGGGCAATGGCACCGTCATGGGTGCTGAACTCCGCCATGTCCTCGCCACTCTGGGAGA GAAGATGAAGGAGGAAGAGGTAGAAGCGTTGCTGGCAGGCCAGGAGGACTCCAATGGCTGCATCAACTAT GAAGGTACAGCCAGCCTCACCTCATTAAGGAGAGATTCATTTTCAACTTTATTTTCCTCTTCTCTATTGA CATATATGACTTATTATAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201424 representing BC059087
Red=Cloning site Green=Tags(s) MSFSADQIADFKEAFLLFDRTGECKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFL PMMQAISNNKDQGGYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALLAGQEDSNGCINY EGTASLTSLRRDSFSTLFSSSLLTYMTYYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | BC059087 |
ORF Size | 510 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC059087.1 |
RefSeq Size | 649 bp |
RefSeq ORF | 512 bp |
Locus ID | 17901 |
MW | 23.8 kDa |
Gene Summary | Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two non-phosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207061 | Myl1 (untagged) - Mouse myosin, light polypeptide 1 (cDNA clone MGC:70067 IMAGE:30139109), (10ug) |
CNY 3,230.00 |
|
MG201424 | Myl1 (tGFP-tagged) - Mouse myosin, light polypeptide 1 (cDNA clone MGC:70067 IMAGE:30139109) |
CNY 2,090.00 |
|
MR201424L3 | Lenti ORF clone of Myl1 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 1 (cDNA clone MGC:70067 IMAGE:30139109) |
CNY 3,800.00 |
|
MR201424L4 | Lenti ORF clone of Myl1 (mGFP-tagged) - Mouse myosin, light polypeptide 1 (cDNA clone MGC:70067 IMAGE:30139109) |
CNY 3,800.00 |