United States
Germany
Japan
United Kingdom
China
Polr3g (BC030063) Mouse Tagged Lenti ORF Clone
CAT＃: MR201232L4
- LentiORF®
Lenti ORF clone of Polr3g (mGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833)
"BC030063" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | RPC7, RPC32 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR201232).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC030063 |
| ORF Size | 480 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC030063, AAH30063 |
| RefSeq Size | 753 bp |
| RefSeq ORF | 482 bp |
| Locus ID | 67486 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206910 | Polr3g (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833), (10ug) |
CNY 3,230.00 |
|
| MG201232 | Polr3g (tGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) |
CNY 2,850.00 |
|
| MR201232 | Polr3g (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) |
CNY 1,200.00 |
|
| MR201232L3 | Lenti ORF clone of Polr3g (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) |
CNY 4,750.00 |
