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Mafk (NM_010757) Mouse Tagged ORF Clone
CAT＃: MR201177
- TrueORF®
Mafk (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk)
ORF Plasmid: tGFP
"NM_010757" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW061068; NF-E2; Nfe2u |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201177 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACTAATCCCAAGCCCAACAAGGCATTGAAGGTCAAGAAGGAGGCGGGTGAGAACGCCCCTGTTC TTAGCGATGATGAGCTGGTGTCCATGTCAGTGCGGGAGCTAAACCAGCACCTGCGGGGGCTCACCAAGGA GGAGGTCACTCGGCTGAAGCAGCGGCGGCGCACACTCAAGAACAGAGGCTACGCGGCTAGCTGTCGCATC AAGCGTGTGACACAGAAAGAGGAGCTGGAACGGCAGCGTGTGGAGCTACAGCAGGAGGTGGAGAAGCTGG CTCGAGAGAACAGCAGCATGCGGCTGGAGCTAGATGCCCTGCGCTCCAAGTATGAGGCCCTACAGACCTT CGCTCGCACCGTGGCCCGAGGGCCTGTCACACCCACCAAGGTGGCCACCACCAGTGTCATCACCATCGTC AAATCTGCCGAGCTCTCCTCCACCTCTGTACCCTTCTCAGCCGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201177 protein sequence
Red=Cloning site Green=Tags(s) MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRI KRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVTPTKVATTSVITIV KSAELSSTSVPFSAAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010757 |
| ORF Size | 471 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010757.2, NP_034887.1 |
| RefSeq Size | 2825 bp |
| RefSeq ORF | 471 bp |
| Locus ID | 17135 |
| UniProt ID | Q61827 |
| MW | 17.5 kDa |
| Gene Summary | Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207325 | Mafk (untagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk), (10ug) |
CNY 1,320.00 |
|
| MG201177 | Mafk (tGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk) |
CNY 2,850.00 |
|
| MR201177L1 | Lenti ORF clone of Mafk (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk) |
CNY 3,600.00 |
|
| MR201177L2 | Lenti ORF clone of Mafk (mGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk) |
CNY 4,750.00 |
|
| MR201177L3 | Lenti ORF clone of Mafk (Myc-DDK-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk) |
CNY 4,750.00 |
|
| MR201177L4 | Lenti ORF clone of Mafk (mGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) (Mafk) |
CNY 4,750.00 |
