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Pfn2 (NM_019410) Mouse Tagged ORF Clone
CAT＃: MR200894
- TrueORF®
Pfn2 (Myc-DDK-tagged) - Mouse profilin 2 (Pfn2)
ORF Plasmid: tGFP
"NM_019410" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pfn |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200894 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGTTGGCAGAGCTACGTGGATAACCTGATGTGCGATGGCTGCTGCCAGGAGGCCGCCATTGTCG GCTACTGCGACGCCAAATACGTCTGGGCAGCCACGGCCGGGGGCGTCTTCCAGAGCATCACGCCAGTAGA AATAGATATGATTGTAGGAAAAGACCGGGAAGGTTTCTTTACCAACGGTTTGACTCTTGGAGCAAAGAAG TGTTCTGTGATCAGAGATAGCCTATACGTTGATGGTGACTGCACAATGGACATCCGGACAAAGAGTCAAG GTGGGGAGCCAACATACAACGTTGCTGTTGGCAGGGCCGGGAGAGTCTTGGTCTTTGTAATGGGAAAAGA AGGGGTCCATGGAGGCGGATTGAATAAGAAGGCATACTCAATGGCAAAATACTTGAGAGACTCTGGGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200894 protein sequence
Red=Cloning site Green=Tags(s) MAGWQSYVDNLMCDGCCQEAAIVGYCDAKYVWAATAGGVFQSITPVEIDMIVGKDREGFFTNGLTLGAKK CSVIRDSLYVDGDCTMDIRTKSQGGEPTYNVAVGRAGRVLVFVMGKEGVHGGGLNKKAYSMAKYLRDSGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019410 |
| ORF Size | 423 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019410.3 |
| RefSeq Size | 2020 bp |
| RefSeq ORF | 423 bp |
| Locus ID | 18645 |
| UniProt ID | Q9JJV2 |
| MW | 15 kDa |
| Gene Summary | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The actin binding protein profilin 2 is a novel regulator of iron homeostasis
,Luscieti, S;Galy, B;Gutierrez, L;Reinke, M;Couso, J;Shvartsman, M;Di Pascale, A;Witke, W;Hentze, MW;Pilo Boyl, P;Sanchez, M;,
Blood
,PubMed ID 28774878
[PFN2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201857 | Pfn2 (untagged) - Mouse profilin 2 (Pfn2), (10ug) |
CNY 1,200.00 |
|
| MG200894 | Pfn2 (tGFP-tagged) - Mouse profilin 2 (Pfn2) |
CNY 2,850.00 |
|
| MR200894L3 | Lenti ORF clone of Pfn2 (Myc-DDK-tagged) - Mouse profilin 2 (Pfn2) |
CNY 4,750.00 |
|
| MR200894L4 | Lenti ORF clone of Pfn2 (mGFP-tagged) - Mouse profilin 2 (Pfn2) |
CNY 4,750.00 |
