Hspa5 (NM_022310) Mouse Tagged ORF Clone
CAT#: MG227570
- TrueORF®
Hspa5 (tGFP-tagged) - Mouse heat shock protein 5 (Hspa5) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_022310" in other vectors (5)
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CNY 5,230.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AL022860; AU019543; baffled; Bip; D2Wsu17e; D2Wsu141e; Grp78; Hsce70; mBiP; SEZ-7; Sez7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227570 representing NM_022310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGTTCACTGTGGTGGCGGCGGCGTTGCTGCTGCTGGGCGCGGTGCGGGCCGAGGAGGAGGACA AGAAGGAGGATGTGGGCACGGTGGTCGGCATCGACTTGGGGACCACCTATTCCTGCGTCGGTGTGTTCAA GAACGGCCGCGTGGAGATCATAGCCAACGATCAGGGCAACCGCATCACGCCGTCGTATGTGGCCTTCACT CCTGAAGGGGAGCGTCTGATTGGCGATGCGGCCAAGAACCAACTCACGTCCAACCCCGAGAACACGGTCT TCGATGCCAAGCGCCTCATCGGACGCACTTGGAATGACCCTTCGGTGCAGCAGGACATCAAGTTCTTGCC ATTCAAGGTGGTTGAAAAGAAAACTAAACCGTACATTCAAGTTGATATTGGAGGTGGGCAAACCAAGACA TTTGCCCCAGAAGAAATTTCTGCCATGGTTCTCACTAAAATGAAGGAGACTGCTGAGGCGTATTTGGGAA AGAAGGTTACCCATGCAGTTGTTACTGTACCAGCTTACTTCAATGATGCCCAGCGACAAGCAACCAAAGA TGCTGGCACTATTGCTGGACTGAATGTCATGAGGATCATCAATGAGCCTACAGCAGCTGCTATTGCATAT GGCCTGGATAAGAGAGAGGGAGAGAAGAACATCCTTGTGTTTGACCTGGGTGGCGGCACCTTCGATGTGT CTCTTCTCACCATTGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGATACTCACCTGGGTGGGGA AGACTTTGATCAGCGGGTCATGGAACACTTCATCAAGTTGTACAAAAAGAAAACTGGTAAAGATGTTAGG AAAGACAACAGAGCTGTGCAGAAACTCCGGCGTGAGGTAGAAAAGGCTAAGAGAGCCTTGTCTTCTCAGC ATCAAGCAAGGATTGAAATTGAGTCCTTCTTCGAAGGAGAAGACTTCTCAGAGACCCTTACTCGGGCCAA ATTTGAAGAGCTGAACATGGACCTGTTCCGCTCTACCATGAAGCCTGTCCAGAAAGTGTTGGAAGACTCT GATCTGAAGAAATCTGATATTGATGAAATTGTTCTGGTTGGTGGATCTACTCGAATTCCAAAGATTCAGC AACTGGTGAAAGAGTTCTTCAATGGCAAGGAGCCATCCCGTGGCATAAACCCCGATGAGGCTGTAGCCTA TGGTGCCGCTGTCCAGGCTGGTGTCCTCTCTGGTGATCAGGATACAGGTGATCTGGTACTGCTTGATGTT TGTCCCCTTACACTTGGTATTGAAACTGTGGGAGGAGTCATGACAAAACTGATTCCAAGGAACACTGTGG TACCCACCAAGAAGTCTCAGATCTTCTCCACGGCTTCCGATAATCAGCCAACTGTAACAATCAAGGTCTA TGAAGGTGAACGACCCCTAACAAAAGACAATCATCTTCTGGGTACATTTGATCTGACTGGAATTCCTCCT GCTCCCCGTGGAGTTCCCCAGATTGAAGTCACTTTTGAGATAGATGTTAATGGTATTCTCCGAGTGACAG CTGAAGACAAAGGTACAGGAAACAAAAACAAAATCACAATTACCAATGACCAAAACCGCCTGACACCTGA AGAAATTGAAAGGATGGTTAATGATGCTGAGAAGTTTGCTGAGGAAGACAAAAAGCTCAAAGAGCGCATT GACACCAGGAATGAATTGGAAAGCTATGCTTATTCTCTCAAGAACCAGATTGGAGATAAAGAAAAGCTGG GAGGTAAACTTTCTTCTGAAGATAAAGAAACCATGGAAAAAGCTGTAGAGGAAAAGATTGAATGGCTGGA AAGCCACCAGGATGCGGACATTGAAGACTTTAAAGCCAAAAAGAAGGAACTAGAAGAAATTGTTCAGCCA ATTATCAGCAAACTCTATGGAAGTGGAGGCCCTCCCCCAACTGGTGAAGAGGATACATCAGAAAAAGATG AGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227570 representing NM_022310
Red=Cloning site Green=Tags(s) MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFT PEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKT FAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAY GLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVR KDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDS DLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDV CPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPP APRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERI DTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQP IISKLYGSGGPPPTGEEDTSEKDEL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022310 |
ORF Size | 1965 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022310.3, NP_071705.3 |
RefSeq Size | 2616 bp |
RefSeq ORF | 1968 bp |
Locus ID | 14828 |
UniProt ID | P20029 |
Gene Summary | Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202686 | Hspa5 (untagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 2, (10ug) |
CNY 7,328.00 |
|
MG209794 | Hspa5 (tGFP-tagged) - Mouse heat shock 70kD protein 5 (glucose-regulated protein) (Hspa5) |
CNY 5,230.00 |
|
MR227570 | Hspa5 (Myc-DDK-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 2 |
CNY 7,800.00 |
|
MR227570L3 | Lenti ORF clone of Hspa5 (Myc-DDK-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 2 |
CNY 6,650.00 |
|
MR227570L4 | Lenti ORF clone of Hspa5 (mGFP-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 2 |
CNY 6,650.00 |