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Dab2ip (NM_001114125) Mouse Tagged ORF Clone
CAT＃: MG227510
- TrueORF®
Dab2ip (tGFP-tagged) - Mouse disabled homolog 2 (Drosophila) interacting protein (Dab2ip) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001114125" in other vectors (4)
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CNY 12,450.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310011D08Rik; AI480459; Aip1; mKIAA1743 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227510 representing NM_001114125
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGACTCCCTCCTGGACCCAGGCGACTCCTACGAGTCGCCCCAAGAAAGGCCGGGCTCCCGGC GGAGCCTTCCCGGCAGCATGTCAGAGAAAAACCCCAGCATGGAGCCCTCGGCTTCAACCCCGTTCCGGGT CACGGGCTTCCTCAGCCGCCGCCTCAAGGGCTCCATCAAGCGCACCAAGAGCCAGCCCAAACTGGACCGC AACCACAGCTTCCGCCACATCCTGCCGGGGTTCCGGAGCGCAGCCGCCGCCGCCGCGGACAATGAGAGGT CCCATCTGATGCCAAGGCTGAAGGAGTCTCGGTCACACGAGTCCCTGCTCAGCCCCAGCAGCGCAGTGGA GGCCCTGGACCTCAGCATGGAGGAGGAGGTGATTATCAAGCCCGTTCACAGCAGCATCCTGGGTCAGGAC TACTGCTTCGAGGTGACAACATCATCAGGAAGCAAGTGTTTTTCCTGCCGGTCAGCCGCTGAGCGCGATA AGTGGATGGAGAACCTGAGGCGAGCAGTGCACCCCAACAAGGACAACAGCCGGCGTGTGGAGCATATCCT GAAGCTGTGGGTGATTGAGGCCAAGGATCTGCCGGCCAAGAAGAAGTATCTATGTGAACTGTGCCTGGAC GATGTGCTGTATGCCCGTACCACAAGCAAGCTCAAGACGGACAATGTCTTCTGGGGAGAGCACTTTGAGT TCCATAACCTGCCGCCTCTACGCACAGTCACTGTGCACCTGTATCGGGAGACTGACAAGAAAAAGAAAAA GGAACGCAACAGCTACCTGGGCCTGGTGAGCCTGCCTGCCGCCTCTGTGGCTGGGCGGCAGTTTGTGGAG AAGTGGTACCCAGTGGTGACACCCAACCCCAAGGGTGGCAAAGGCCCTGGGCCCATGATCCGAATCAAGG CACGCTACCAGACCGTCAGCATCTTGCCTATGGAGATGTACAAGGAGTTTGCGGAGCACATCACTAACCA CTACCTGGGGCTGTGCGCAGCCCTGGAACCCATCCTCAGTGCCAAGACCAAGGAGGAGATGGCGTCGGCT CTGGTGCACATCCTGCAGAGCACGGGAAAGGTGAAGGACTTTCTAACAGACCTGATGATGTCAGAGGTGG ACCGCTGTGGGGACAATGAGCACCTCATCTTCCGGGAGAACACACTGGCCACCAAGGCCATCGAGGAATA CCTCAAACTTGTGGGCCAGAAGTACCTGCAGGACGCACTAGGTGAGTTCATCAAAGCTCTGTATGAGTCA GATGAAAATTGTGAAGTGGACCCAAGCAAGTGCTCATCCGCTGACCTCCCTGAGCACCAGGGCAACCTCA AGATGTGCTGTGAGCTGGCCTTCTGCAAGATCATCAACTCCTACTGCGTCTTCCCACGGGAGCTTAAGGA GGTGTTCGCCTCATGGCGGCAGGAGTGTAGCAGCCGAGGCCGGCCAGATATCAGTGAACGGCTCATCAGC GCCTCCCTCTTCCTTCGCTTCCTGTGCCCTGCCATCATGTCACCCTCGCTCTTCAACCTGCTTCAGGAGT ATCCTGACGACCGCACGGCTCGCACCCTCACGCTCATTGCCAAAGTCACCCAGAACCTGGCCAACTTTGC CAAGTTTGGCAGCAAGGAAGAATACATGTCCTTCATGAACCAGTTCCTGGAGCACGAGTGGACCAACATG CAGCGCTTCCTGTTGGAGATCTCCAACCCCGAGACCCTTTCCAACACAGCAGGCTTCGAGGGCTACATAG ACCTGGGCCGGGAGCTCTCTAGCCTGCACTCCCTGCTCTGGGAAGCTGTCAGCCAGCTTGATCAGAGCGT TGTGTCGAAGCTGGGACCTCTGCCTCGTATCCTGAGGGATGTCCACACAGCACTGAGCACTCCTGGCAGT GGGCAGCTCCCTGGCACCAATGACCTGGCCTCCACCCCGGGCTCCGGCAGCAGCAGCGTCTCTGCTGGGC TTCAGAAGATGGTGATTGAAAATGACCTCTCTGGTCTGATAGATTTCACCCGGTTACCGTCTCCAACCCC CGAAAACAAGGACTTGTTTTTTGTCACAAGGTCCTCCGGGGTCCAGCCTTCACCTGCCCGCAGCTCAAGC TACTCAGAAGCCAATGAACCTGACCTGCAGATGGCCAATGGCAGCAAGAGCCTGTCCATGGTGGACCTCC AGGACGCCCGCACGCTGGATGGGGAGGCAGGTTCCCCAGTGGGCCCAGACGCCCTACCTGCTGACGGGCA GGTGCCTGCGACTCAGCTGCTGGCTGGGTGGCCAGCCAGGGCAGCCCCAGTGAGCCTGGCAGGATTGGCC ACAGTGCGGCGGGCAGTGCCAACACCAACCACACCAGGCACCTCCGAGGGTGCACCAGGACGGCCCCAGT TGTTGGCCCCACTTTCCTTCCAGAATCCTGTGTACCAGATGGCGGCCGGCCTGCCACTGTCACCCCGTGG CCTTGGTGACTCAGGCTCTGAAGGCCACAGCTCCCTGAGCTCTCACAGCAACAGTGAAGAGCTGGCAGCC GCTGCCAAACTAGGAAGTTTCAGCACTGCTGCAGAGGAGCTGGCAAGGCGGCCTGGAGAACTGGCACGGA GGCAGATGTCACTGACTGAGAAGGGTGGGCAGCCCACAGTGCCGAGGCAAAATAGTGCCGGTCCCCAGCG GAGGATTGACCAGCCGCCACCGCCACCACCACCACCGCCTCCTGCTCCCCGGGGCAGGACACCTCCTACC CTGCTGAGCACCCTACAGTACCCACGACCCTCAAGTGGAACCCTGGCATCAGCATCCCCCGACTGGGCTG GCCCTGGCACCCGGCTGCGGCAACAGTCCTCCTCCTCCAAGGGAGACAGCCCAGAGCTGAAGCCCCGAGC CATGCACAAGCAGGGCCCTTCACCCGTCAGTCCCAATGCCCTGGACCGCACGGCCGCTTGGCTCTTGACC ATGAACGCGCAGTTGTTAGAAGACGAGGGTCTGGGCCCAGATCCCCCCCACAGGGATAGGCTAAGGAGTA AGGAGGAACTCAGCCAAGCAGAAAAGGATCTGGCAGTGCTACAAGACAAGCTACGGATCTCCACCAAGAA GCTGGAGGAGTATGAGACCCTATTCAAGTGCCAGGAGGAGACGACGCAGAAGCTGGTGCTGGAGTATCAG GCTCGGCTGGAAGAAGGTGAGGAGCGGCTGCGGCGGCAGCAGGAAGACAAGGATATCCAGATGAAAGGCA TCATCAGCAGGTTGATGTCAGTGGAAGAAGAACTGAAGAAGGATCATGCAGAGATGCAAGCAGCTGTAGA TTCCAAACAGAAGATCATCGATGCCCAGGAAAAGCGCATTGCCTCGCTGGATGCTGCCAATGCCCGCCTC ATGAGTGCCCTCACACAGCTGAAAGAGAGTATGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227510 representing NM_001114125
Red=Cloning site Green=Tags(s) MEPDSLLDPGDSYESPQERPGSRRSLPGSMSEKNPSMEPSASTPFRVTGFLSRRLKGSIKRTKSQPKLDR NHSFRHILPGFRSAAAAAADNERSHLMPRLKESRSHESLLSPSSAVEALDLSMEEEVIIKPVHSSILGQD YCFEVTTSSGSKCFSCRSAAERDKWMENLRRAVHPNKDNSRRVEHILKLWVIEAKDLPAKKKYLCELCLD DVLYARTTSKLKTDNVFWGEHFEFHNLPPLRTVTVHLYRETDKKKKKERNSYLGLVSLPAASVAGRQFVE KWYPVVTPNPKGGKGPGPMIRIKARYQTVSILPMEMYKEFAEHITNHYLGLCAALEPILSAKTKEEMASA LVHILQSTGKVKDFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEEYLKLVGQKYLQDALGEFIKALYES DENCEVDPSKCSSADLPEHQGNLKMCCELAFCKIINSYCVFPRELKEVFASWRQECSSRGRPDISERLIS ASLFLRFLCPAIMSPSLFNLLQEYPDDRTARTLTLIAKVTQNLANFAKFGSKEEYMSFMNQFLEHEWTNM QRFLLEISNPETLSNTAGFEGYIDLGRELSSLHSLLWEAVSQLDQSVVSKLGPLPRILRDVHTALSTPGS GQLPGTNDLASTPGSGSSSVSAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPARSSS YSEANEPDLQMANGSKSLSMVDLQDARTLDGEAGSPVGPDALPADGQVPATQLLAGWPARAAPVSLAGLA TVRRAVPTPTTPGTSEGAPGRPQLLAPLSFQNPVYQMAAGLPLSPRGLGDSGSEGHSSLSSHSNSEELAA AAKLGSFSTAAEELARRPGELARRQMSLTEKGGQPTVPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPT LLSTLQYPRPSSGTLASASPDWAGPGTRLRQQSSSSKGDSPELKPRAMHKQGPSPVSPNALDRTAAWLLT MNAQLLEDEGLGPDPPHRDRLRSKEELSQAEKDLAVLQDKLRISTKKLEEYETLFKCQEETTQKLVLEYQ ARLEEGEERLRRQQEDKDIQMKGIISRLMSVEEELKKDHAEMQAAVDSKQKIIDAQEKRIASLDAANARL MSALTQLKESMH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001114125 |
| ORF Size | 3396 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001114125.1, NP_001107597.1 |
| RefSeq Size | 5388 bp |
| RefSeq ORF | 3399 bp |
| Locus ID | 69601 |
| UniProt ID | Q3UHC7 |
| Gene Summary | Functions as a scaffold protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Involved in several processes such as innate immune response, inflammation and cell growth inhibition, apoptosis, cell survival, angiogenesis, cell migration and maturation. Plays also a role in cell cycle checkpoint control; reduces G1 phase cyclin levels resulting in G0/G1 cell cycle arrest. Mediates signal transduction by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF), interferon (IFN) or lipopolysaccharide (LPS). Modulates the balance between phosphatidylinositol 3-kinase (PI3K)-AKT-mediated cell survival and apoptosis stimulated kinase (MAP3K5)-JNK signaling pathways; sequesters both AKT1 and MAP3K5 and counterbalances the activity of each kinase by modulating their phosphorylation status in response to proinflammatory stimuli. Acts as a regulator of the endoplasmic reticulum (ER) unfolded protein response (UPR) pathway; specifically involved in transduction of the ER stress-response to the JNK cascade through ERN1. Mediates TNF-alpha-induced apoptosis activation by facilitating dissociation of inhibitor 14-3-3 from MAP3K5; recruits the PP2A phosphatase complex which dephosphorylates MAP3K5 on 'Ser-966', leading to the dissociation of 13-3-3 proteins and activation of the MAP3K5-JNK signaling pathway in endothelial cells. Mediates also TNF/TRAF2-induced MAP3K5-JNK activation, while it inhibits CHUK-NF-kappa-B signaling. Acts a negative regulator in the IFN-gamma-mediated JAK-STAT signaling cascade by inhibiting smooth muscle cell (VSMCs) proliferation and intimal expansion, and thus, prevents graft arteriosclerosis (GA). Acts as a GTPase-activating protein (GAP) for the ADP ribosylation factor 6 (ARF6) and Ras. Promotes hydrolysis of the ARF6-bound GTP and thus, negatively regulates phosphatidylinositol 4,5-bisphosphate (PIP2)-dependent TLR4-TIRAP-MyD88 and NF-kappa-B signaling pathways in endothelial cells in response to lipopolysaccharides (LPS). Binds specifically to phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 3-phosphate (PtdIns3P). In response to vascular endothelial growth factor (VEGFA), acts as a negative regulator of the VEGFR2-PI3K-mediated angiogenic signaling pathway by inhibiting endothelial cell migration and tube formation. In the developing brain, promotes both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex in a glial-dependent locomotion process. Probable downstream effector of the Reelin signaling pathway; promotes Purkinje cell (PC) dendrites development and formation of cerebellar synapses. Functions also as a tumor suppressor protein in prostate cancer progression; prevents cell proliferation and epithelial-to-mesenchymal transition (EMT) through activation of the glycogen synthase kinase-3 beta (GSK3B)-induced beta-catenin and inhibition of PI3K-AKT and Ras-MAPK survival downstream signaling cascades, respectively.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223689 | Dab2ip (untagged) - Mouse disabled homolog 2 (Drosophila) interacting protein (Dab2ip), transcript variant 1, (10ug) |
CNY 14,540.00 |
|
| MR227510 | Dab2ip (Myc-DDK-tagged) - Mouse disabled homolog 2 (Drosophila) interacting protein (Dab2ip), transcript variant 1 |
CNY 11,310.00 |
|
| MR227510L3 | Lenti ORF clone of Dab2ip (Myc-DDK-tagged) - Mouse disabled homolog 2 (Drosophila) interacting protein (Dab2ip), transcript variant 1 |
CNY 13,210.00 |
|
| MR227510L4 | Lenti ORF clone of Dab2ip (mGFP-tagged) - Mouse disabled homolog 2 (Drosophila) interacting protein (Dab2ip), transcript variant 1 |
CNY 13,210.00 |
